ALKALI-CATION YEAST TRANSFORMA

ALKALI-CATION YEAST TRANSFORMA

Synonyms High Efficiency Yeast Transformation Kit

Packaging size: 250 Preps

SKU 112200200
Price:
$293.10
In Stock
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Description

Product Description

Alkali-Cation™ Yeast Transformation Kit is easy to use, robust, and its scalability makes this the method of choice for the high efficiency transformation needed in two hybrid screens.

Application Notes

Alkali-Cation™ Yeast Transformation Kit provides an efficient method for yeast transfomation and has been successfully used to transform linear DNA into Pichia pastoris for protein expression studies.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Transformation of yeast cells

Specifications
SKU 112200200
Alternate Names High Efficiency Yeast Transformation Kit
Application Notes Alkali-Cation™ Yeast Transformation Kit provides an efficient method for yeast transfomation and has been successfully used to transform linear DNA into Pichia pastoris for protein expression studies.
Cells Tested S. cerevisiae, S. pombe, S. albicans, many strains
Components Sterile TE Buffer pH 7.5 |Lithium/Cesium Acetate |Carrier DNA 10 mg/mL Sterile |Sterile Solution Histamine |TE/Cation MIXX |PEG |Sterile Solution SOS
Format All necessary reagents
Applications Transformation of yeast cells
Kit Compatibility GENECLEAN® Kits |MERmaid™ Kits |FASTDNA™ Kits |FASTRNA™ Kits | Other suppliers' kits
Kit or Assay Type Transformation Kits
Materials Required Not Supplied Cultureware |Culture media |Centrifuge |Incubator with shaker |Agar |Vortexer
Pack Size 250 Preps
Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: PVC or other plastic material gloves. Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields. Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
Physical Appearance Solution
Presentation Kit
Product Overview Alkali-Cation™ Yeast Transformation Kit provides all of the reagents required to perform an easy and quick non-electrical method for transforming yeast with linear or plasmid DNA. Host cells are made competent by treatment with a Lithium/Cesium Acetate mixture. The entire procedure may be completed in less than 90 minutes and routinely provides a transformation efficiency of greater than 104 transformants per µg of DNA.
Recommended Alternate EZ-Yeast™ Transformation Kit
Recommended Replacement For 112100200
Research Areas Molecular Biology |Microbiology |Cell Biology |Biochemistry |Genomics |Gene Expression |Gene Regulation |Oncology
Safety Symbol GHS07 |GHS08
Sample Throughput Transformation complete in 90 minutes. Transformants observed after 48-72 hours incubation.
Sample Type Overnight yeast culture or colonies from plates
Sample Volume 500 µL or 3-4 mm colony
Storage and Handling Store at 4 °C.
Trade Name Alkali-Cation™
Unit Definition ≥ 104 transformants/µg plasmid DNA
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.