EZ-YEAST TRANSFORMATION KIT
The EZ-Yeast™ Transformation Kit is designed for high-throughput transformation with efficiencies up to 103 transformants per μg plasmid DNA. Perfect for a quick analysis of potentially positive two-hybrid colonies, the EZ-Yeast™ Transformation Kit does not require fully competent cells prior to transformation. Simply remove a 3-4 mm colony or 0.5 mL overnight culture to a new tube, add vector and carrier DNA, incubate for 30 minutes and plate on selective media.
The EZ-Yeast Transformation Kit is designed for high throughput transformations. It is ideal for cases where a large number of transformations must be performed, but only a few transformants are needed.
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Transformation of yeast cells
|Alternate Names||Yeast 2-hybrid reporter kit|
|Application Notes||The EZ-Yeast Transformation Kit is designed for high throughput transformations. It is ideal for cases where a large number of transformations must be performed, but only a few transformants are needed.|
|Cells Tested||S. cerevisiae, S. pombe, S. albicans, many strains|
|Components||EZ-Transformation Solution |Carrier DNA|
|Format||Single Reagent Kit plus DNA|
|Applications||Transformation of yeast cells|
|Kit Compatibility||GENECLEAN® Kits |MERmaid™ Kits |FASTDNA™ Kits |FASTRNA™ Kits | Other suppliers' kits|
|Kit or Assay Type||Transformation Kits|
|Materials Required Not Supplied||Cultureware |Culture media |Centrifuge |Incubator with shaker |Agar |Vortexer|
|Personal Protective Equipment||Personal-protective-equipment: Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.|
|Product Overview||The EZ-Yeast™ Transformation Kit is designed for high throughput transformations. It is ideal for cases where a large number of transformations must be performed, but only a few transformants are needed. The EZ-Yeast™ Transformation Kit does not require preparing competent cells prior to transformation, simply resuspend yeast cells from an overnight culture or a fresh plate in EZ Transformation solution. Add the vector and carrier DNA, incubate at 42°C for 30 min. and plate on selective media. Lower temperatures can also be used. The EZ-Yeast™ Transformation Kit is ideal for the simultaneous transformation of two-hybrid reporter strains with bait and library vectors when processing putative positive clones. It is capable of achieving efficiencies of up to 1000 colonies per μg DNA. For high efficiency transformation use the Alkali Cation Yeast Transformation Kit (cat# 2200-200) or the Yeast Spheroplast Transformation Kit (Cat # 2210-200)|
|Recommended Alternate||Alkali-Cation™ Yeast Transformation Kit|
|Recommended Replacement For||112200200|
|Research Areas||Molecular Biology |Microbiology |Cell Biology |Biochemistry |Genomics |Gene Expression |Gene Regulation |Oncology|
|Sample Throughput||Transformation complete in 30 minutes. Transformants observed after 3 days incubation.|
|Sample Type||Overnight yeast culture or colonies from plates|
|Sample Volume||500 µL or 3-4 mm colony|
|Storage and Handling||Store EZ-Transformation Solution at Room temperature (15-30 °C). Store Carrier DNA at 4°C.|
|Unit Definition||≤ 103 transformants/µg plasmid DNA|