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RPM YEAST PLASMID ISOLATION KI

RPM YEAST PLASMID ISOLATION KI

Synonyms Yeast Plasmid Miniprep Purification Kit

Packaging size: 100 Preps

SKU 112069400
Price:
$434.90
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Description

Product Description

The RPM™ Yeast Plasmid Isolation Kit allows isolation of plasmid DNA from yeast cells. It uses a GLASSMILK purification process in conjunction with SPIN modules to increase yield and achieve optimal DNA quality. The isolated DNA can be used for screening and bacterial transformations.

Application Notes

The Yeast Plasmid Isolation Kit is a Rapid Pure Miniprep Kit designed for yeast cells. The plasmid isolated from yeast is suitable for PCR and bacterial transformation. The same kit can be used to isolate plasmid from bacterial transformants for use in any downstream application (PCR, sequencing, restriction enzyme digestion, labeling, etc). Plasmid DNA mini-preps from yeast. Rescue positive prey plasmids after a yeast two-hybrid or a yeast one-hybrid screen.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Isolation of yeast plasmid DNA

Specifications
SKU 112069400
Application Notes The Yeast Plasmid Isolation Kit is a Rapid Pure Miniprep Kit designed for yeast cells. The plasmid isolated from yeast is suitable for PCR and bacterial transformation. The same kit can be used to isolate plasmid from bacterial transformants for use in any downstream application (PCR, sequencing, restriction enzyme digestion, labeling, etc). Plasmid DNA mini-preps from yeast. Rescue positive prey plasmids after a yeast two-hybrid or a yeast one-hybrid screen.
Components Yeast Lysing Matrix |Alkaline Lysis Solution |Neutralizing Solution |GLASSMILK™ Spin Buffer |Wash Solution Concentrate |SPIN Filters |Catch Tubes |Pre-Lysis Buffer
Format Geneclean® GLASSMILK™ slurry plus SPIN filter
Applications Isolation of yeast plasmid DNA
Kit or Assay Type Plasmid DNA from yeast isolation kit
Materials Required Not Supplied Benchtop Microcentrifuge |Vortexer |FastPrep Instrument or other high-speed reciprocating homogenizer (optional)
Molecule Enriched DNA
Pack Size 100 Preps
Personal Protective Equipment Personal-protective-equipment: Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
pH Alkaline Lysis Solution pH Range: 12.6-12.8 |Neutralizing Solution pH Range: 5.5-5.9 |Wash Solution Concentrate pH Range: 7.4-7.7 |Pre-Lysis Buffer pH Range: 6.6-7.0
Physical Appearance Solution |Slurry | Spin filter module
Preparation Method Before first use, add the 56 mL of 200 proof (100% ethanol) to the Wash Solution Concentrate (56 mL) and mix well. Do not use denatured alcohol because it can cause precipitation of salts. Label the container. Store tightly capped at 15°-30°C.
Presentation Kit
Product Overview The RPM™ Yeast Plasmid Isolation Kit for the isolation of plasmid DNA from yeast is a RapidPure™ Miniprep Kit designed for yeast cells. The plasmid isolated from yeast is suitable for PCR and bacterial transformation. This kit is ideal for screening yeast transformants obtained from two-hybrid screens.
Research Areas Molecular Biology |Microbiology |Cell Biology |Biochemistry |Genomics |Environmental and Ecology
Safety Symbol GHS07 |GHS05
Sample Type Yeast Plasmid Prep
Sample Volume 1.5 ml of yeast culture
Storage and Handling Store at room temperature (15-30 °C).
Unit Definition Yields up to 10 μg of plasmid DNA Per 1.5 ml overnight culture typical
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.