G NOME DNA KIT UN3082-III, 9

G NOME DNA KIT UN3082-III, 9

Synonyms DNA Purification

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SKU 112010400-CF
Price:
$125.95
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Description

Product Description

The GNOME® DNA Isolation kit is used to quickly and efficiently isolate high molecular weight genomic DNA from cells and tissues of any type, including bacteria, yeast, plant, and animal cells. Each preparation with the GNOME® kit yields up to 100 µg of genomic DNA. The DNA isolated by the GNOME¨ procedure is suitable for restriction enzyme digestion or PCR amplification in as little as 1 hour after cell lysis.

Application Notes

The GNOME® DNA Isolation Kit is a rapid and efficient method for isolation of high molecular weight genomic DNA from bacteria, plant, yeast, blood cells and other mammalian cells and tissues of all types. No organic extractions are required, and up to 100 μg of DNA can be isolated per prep.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Isolation of high molecular weight DNA from a variety of sources

Specifications
SKU 112010400-CF
Alternate Names DNA Purification
Application Notes The GNOME® DNA Isolation Kit is a rapid and efficient method for isolation of high molecular weight genomic DNA from bacteria, plant, yeast, blood cells and other mammalian cells and tissues of all types. No organic extractions are required, and up to 100 μg of DNA can be isolated per prep.
Brand GNOME®
Components Cell Lysis/Denaturing solution | RNase Mixx | Protease Mixx | Salt out Mixture | Cell Suspension solution.
Format All necessary reagents
Applications Isolation of high molecular weight DNA from a variety of sources
Kit or Assay Type Genomic DNA Pruification Kit
Materials Required Not Supplied Microcentrifuge that can freely spin 2.0 ml tubes |Microcentrifuge tubes (2.0 ml and/or 1.5 ml) |Sterile 15 ml conical tubes for DNA binding |Rotator or low-speed vortex |100% Ethanol
Molecule Enriched DNA
Personal Protective Equipment Personal-protective-equipment: Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
pH Cell Lysis/Denaturing Solution: pH range= 6.2-6.9 |Salt-out Mixture: pH range= 6.0-6.8 |Cell Suspension Solution: pH range= 8.0-8.2
Physical Appearance Solution
Preparation Method Sample preparation protocols provided in kit insert. The yield of genomic DNA and the speed of the protocol are greatly enhanced through the use of the FastPrep®System.
Presentation Kit
Product Families Description The GNOME™ DNA Isolation Kit is used to quickly and efficiently isolate high molecular weight genomic DNA from whole blood, cells, and tissues of any type, including bacteria, yeast, plant, and animal cells. Each preparation with the GNOME™ DNA kit yields up to 100 μg of genomic DNA. The DNA isolated by the GNOME™ procedure is suitable for restriction enzyme digestion or PCR amplification in as little as 1 hour after cell lysis. The GNOME™ DNA kit is available in 2 sizes to suit your needs: 25 or 100 preparations/kit. The GNOME™ DNA kit utilizes RNase Mixx to eliminate RNA immediately after lysis, and Protease Mixx to rapidly degrade cellular proteins. This is followed by a proprietary “salting-out” technique which precludes the need for phenol, chloroform, or other organic extractions. The DNA can be spooled, removed with forceps, or centrifuged and dissolved in TE. The GNOME™ DNA kit contains the reagents to homogenize your cells or tissue and purify high molecular weight DNA. Ethanol is the only reagent not supplied in the kit.
Product Overview The GNOME® kit utilizes RNase Mixx to eliminate RNA immediately after lysis, and Protease Mixx to rapidly degrade cellular proteins. This is followed by a proprietary “salting-out” technique which precludes the need for phenol, chloroform, or other organic extractions. The DNA can either be spooled, removed with forceps, or centrifuged and dissolved in TE.
Recommended Replacement For 116540600
Research Areas Molecular Biology |Plant Biology |Cell Biology |Biochemistry |Genomics |Environmental and Ecology
Safety Symbol GHS05 |GHS07 |GHS08
Sample Type Plant |Bacteria |Yeast |Blood |Tissue
Sample Volume 20-50 mg wet weight bacterial cell pellet | up to 250 mg plant tissue |2-5 x 107 nucleated white blood cells |50-250 mg mammalian tissue |~1-5 x 108 yeast cell pellet
Storage and Handling All GNOME® DNA Isolation Kits are shipped at ambient temperatures. Store all reagents at room temperature (15-30 °C).
Trade Name GNOME™
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.