RNAID SPIN KIT--N/H
RNaid® SPIN KIT provides a simple and convenient way to isolate total RNA from agarose and polyacrylamide gels and from solutions. It Purify RNA fragments from 75 bases to 10 kb.
The RNaid® Spin Kit is designed to isolate and purify RNA (75 bases to 10 kb) from native and denaturing agarose and polyacrylamide gels. Removal of unincorporated nucleotides, buffer salts, and other reagents from enzyme reactions. The purified RNA is ready for reverse transcription and PCR, labeling and microarray analysis, and other common applications.
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Isolate and purify RNA from native and denaturing agarose and polyacrylamide gels and from solutions
|Alternate Names||RNA Clean-Up Kit|
|Application Notes||The RNaid® Spin Kit is designed to isolate and purify RNA (75 bases to 10 kb) from native and denaturing agarose and polyacrylamide gels. Removal of unincorporated nucleotides, buffer salts, and other reagents from enzyme reactions. The purified RNA is ready for reverse transcription and PCR, labeling and microarray analysis, and other common applications.|
|Components||DEPC-Treated Water |RNA Binding Salt |RNA Wash Concentrate |RNAMATRIX® |10% Acetic Acid |SPIN Filters |Catch Tubes|
|Format||RNAMATRIX™ slurry plus SPIN filter|
|Applications||Isolate and purify RNA from native and denaturing agarose and polyacrylamide gels and from solutions|
|Kit or Assay Type||RNA purification kit|
|Materials Required Not Supplied||Microcentrifuge that can freely spin 2.0 ml tubes |Microcentrifuge tubes (2.0 ml and 1.5 ml) |Rotator or low-speed vortex |Gel Electrophoresis Apparatus |Power Source |Sterile water |TAE Buffer |100% Ethanol|
|Pack Size||200 Preps|
|Personal Protective Equipment||Personal-protective-equipment: Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.|
|Physical Appearance||Solution |Slurry | Spin filter module|
|Preparation Method||Before first use, add the 120 mL of 200 proof (100% ethanol) to the RNA Wash Concentrate and mix well. Do not use denatured alcohol because it can cause precipitation of salts. Label the container. Store tightly capped at 15°-30°C.|
|Product Overview||The RNaid™ SPIN Kit Isolates and Purifies RNA from Agarose or Polyacrylamide Gels, from Solutions, and from Unincorporated Radioactive Label.|
|Recommended Alternate||RNaid™ Kit|
|Recommended Replacement For||111007200|
|Research Areas||Molecular Biology |Plant Biology |Cell Biology |Biochemistry |Genomics |Environmental and Ecology|
|Sample Type||Agarose gels |polyacrylamide gels |solutions |enzymatic reactions|
|Sample Volume||Up to ~100 mg gel slice or up to ~100 µl DNA solution|
|Storage and Handling||Store at room temperature (15-30 °C).|
|Unit Definition||One µL of RNAMATRIX™ will bind one µg of RNA.|