MERmaid® Spin Kit
Product Description
MERmaid® SPIN Kit is designed for rapid purification of small MW single-stranded or double-stranded DNA (10 bp to 200 bp) from agarose or polyacrylamide gels, nucleotides, salts, proteins, or other undesirable components of a mixture. This kit combines the advantages of silica binding with a convenient spin column format, eliminating the need for expensive resins or toxic organic compounds such as phenol and chloroform.
Application Notes
The MERmaid SPIN Kit is designed for purifying small MW DNA away from gel material, nucleotides, salts, proteins, or other undesirable components of a mixture. Single-stranded or double-stranded DNA oligomers or PCR products of 10-200 nucleotides in length are readily purified from agarose and polyacrylamide gels or from solutions. Purified DNA can be used in enzymatic reactions, conventional or automated sequencing, cloning and microarray analysis.
Usage Statement
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications
Rapid purification of DNA
| SKU | 111105200-CF |
| Alternate Names | Purification of small molecular weight DNA |
| Application Notes | The MERmaid SPIN Kit is designed for purifying small MW DNA away from gel material, nucleotides, salts, proteins, or other undesirable components of a mixture. Single-stranded or double-stranded DNA oligomers or PCR products of 10-200 nucleotides in length are readily purified from agarose and polyacrylamide gels or from solutions. Purified DNA can be used in enzymatic reactions, conventional or automated sequencing, cloning and microarray analysis. |
| Base Catalog Number | 1105200 |
| Brand | MERmaid™ |
| Components | GLASSFOG™ SPIN Bind Solution |MERmaid™ SPIN High Salt Wash |MERmaid™ SPIN Ethanol Wash Concentrate |MERmaid™Elution Solution |50x Electrophoresis Buffer |BioGel™ |MERmaid™ SPIN Filters |Elution Catch Tubes |Label Block. |
| Foreign Activity | DNase (both exo and endo) and RNase not detected |
| Format | GLASSFOG™ slurry plus SPIN filter |
| Applications | Rapid purification of DNA |
| Kit or Assay Type | DNA purification kit |
| Materials Required Not Supplied | 100% ethanol |Distilled water |Microcentrifuge |Vortexer |Water bath or heat block |
| Molecule Enriched | DNA |
| Personal Protective Equipment | Personal-protective-equipment: Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice. |
| Physical Appearance | Solution |Slurry | Spin filter module |
| Preparation Method | Before first use, add the 22.5 mL of 200 proof (100% ethanol) to the MERmaid™ SPIN Ethanol Wash Concentrate (2.5 mL) and mix well. Do not use denatured alcohol because it can cause precipitation of salts. Label the container. Store tightly capped at 15°-30°C. |
| Presentation | Kit |
| Product Families Description | MERmaid® SPIN Kit is designed for rapid purification of small MW single-stranded or double-stranded DNA (10 bp to 200 bp) from agarose or polyacrylamide gels, nucleotides, salts, proteins, or other undesirable components of a mixture. This kit combines the advantages of silica binding with a convenient spin column format, eliminating the need for expensive resins or toxic organic compounds such as phenol and chloroform. |
| Product Overview | MERmaid® spin kit contains Glassfog® slurry that allows flexibility of reaction size and volume. This kit helps to recover labeled DNA fragments with ease. Up to 95% recovery of ready-to-use DNA. Fast and convenient procedure. |
| Recommended Replacement For | 111005200 |
| Research Areas | Molecular Biology |Plant Biology |Cell Biology |Biochemistry |Genomics |Environmental and Ecology |
| Safety Symbol | GHS07 |
| Sample Type | Agarose gels |polyacrylamide gels |solutions |enzymatic reactions |
| Sample Volume | Up to ~300 mg gel slice or up to ~300 µl DNA solution |
| Storage and Handling | Store at Room Temperature (15-30 °C). |
| Unit Definition | Each prep will bind up to 10 μg of either ssDNA or dsDNA. |
| Usage Statement | Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |