GENECLEAN TURBO FOR PCR

GENECLEAN TURBO FOR PCR

Synonyms PCR Purification Kit

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SKU 111103200-CF
Price:
$103.45
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Description

Product Description

The GENECLEAN® Turbo for PCR Kit is a specially optimized kit for PCR purification of DNA fragments of sizes 100 bp to 10 kb. This kit uses the same cartridge housing as the GENECLEAN® Turbo Kit. However, it contains a unique membrane optimized for PCR product purification. It purify larger fragments due to less manipulation of bound DNA. Process with either vacuum or centrifugation & save time with patented Turbo filter design.

Application Notes

The GENECLEAN® Turbo for PCR Kit removes primers, nucleotides, enzymes, mineral oil, salts, and other impurities from DNA samples. DNA fragments purified with the GENECLEAN® Turbo for PCR Kit are ready for direct use in all applications including sequencing, microarray analysis, ligation and transformation, restriction digestion and labeling.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Isolation of DNA from PCR reactions; removal of primers; nucleotides; enzymes; mineral oil; salts; and other impurities from DNA samples

Specifications
SKU 111103200-CF
Application Notes The GENECLEAN® Turbo for PCR Kit removes primers, nucleotides, enzymes, mineral oil, salts, and other impurities from DNA samples. DNA fragments purified with the GENECLEAN® Turbo for PCR Kit are ready for direct use in all applications including sequencing, microarray analysis, ligation and transformation, restriction digestion and labeling.
Brand GENECLEAN®
Components GENECLEAN® Turbo for PCR Salt Solution(28 mL) |GENECLEAN® Turbo for PCR Wash Concentrate(6 mL) |GENECLEAN® Turbo for PCR Cartridges(50 cartridges) |GENECLEAN® Turbo for PCR Catch Tubes(50 tubes) |GENECLEAN® Turbo for PCR Elution Solution(3.3 mL)
Format GLASSMILK™ imbedded GENECLEAN® Turbo SPIN column
Applications Isolation of DNA from PCR reactions; removal of primers; nucleotides; enzymes; mineral oil; salts; and other impurities from DNA samples
Kit or Assay Type DNA purification kit
Materials Required Not Supplied Benchtop microcentrifuge |200 proof (100%) ethanol |Distilled Water |Vacuum aspirator (optional) |Vacuum manifold (optional)
Molecule Enriched DNA
Personal Protective Equipment Personal-protective-equipment: Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
Physical Appearance Solution| Spin column
Preparation Method Before first use, add the 54 mL of 200 proof (100% ethanol) to the GENECLEAN® Turbo for PCR Wash Concentrate (6 mL) and mix well. Do not use denatured alcohol because it can cause precipitation of salts. Label the container. Store tightly capped at 15°-30°C.
Presentation Kit
Product Families Description The GENECLEAN® Turbo for PCR Kit is a specially optimized kit for PCR purification of DNA fragments of sizes 100 bp to 10 kb. This kit uses the same cartridge housing as the GENECLEAN® Turbo Kit. However, it contains a unique membrane optimized for PCR product purification. It purify larger fragments due to less manipulation of bound DNA. Process with either vacuum or centrifugation & save time with patented Turbo filter design.
Product Overview The GENECLEAN® Turbo for PCR Kit is a specially optimized kit for the purification of PCR products ranging from 100 bp to 10 kb. It contains Glassmilk® embedded filters in unique Turbo cartridges for maximal efficiency and convenience. Glassmilk® is able to preferentially bind nucleic acids in the presence of high salts and low pH. Since the binding process is specific for nucleic acids, the bound molecules can be separated and purified from impurities by simple washing steps. The DNA is then eluted from the matrix in low salt buffer or water, ready-to-use for downstream applications.
Research Areas Molecular Biology |Plant Biology |Cell Biology |Biochemistry |Genomics |Environmental and Ecology
Safety Symbol GHS07
Sample Type Post amplification PCR reaction mixture (oil-free)
Sample Volume Up to 100 µl PCR reaction solution
Storage and Handling All GENECLEAN® Turbo for PCR Kits are shipped at ambient temperatures. Store all reagents at room temperature (15-30 °C).
Unit Definition Each GENECLEAN® Turbo for PCR Cartridge has a binding capacity of 10 μg of either ssDNA or dsDNA.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.