GENECLEAN SPIN KIT UN1760-III

GENECLEAN SPIN KIT UN1760-III

Synonyms DNA Clean-Up Kit

CAS Number: GUANIDINE THIOCYANATE 593-84-0||Water, Ultra-pure 7732-18-5

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SKU 111101200-CF
Price:
$152.30
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Description

Product Description

The GENECLEAN® SPIN kit simplifies the process of purifying DNA into three easy steps: Bind,Wash and Elute. Ethanol precipitation is never required and purified DNA is immediately ready for a wide variety of downstream applications.

Application Notes

The GENECLEAN® SPIN Kit is used to purify fragments of DNA from 200 bp to 300 kb from solutions and agarose gels. Examples of applications are desalting, elimination of proteins from enzymatic reactions, removal of primers and unincorporated nucleotides from enzymatic reactions, separation of linearized from uncut vector and isolation of PCR products away from genomic DNA.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Examples of applications are desalting; elimination of proteins from enzymatic reactions; removal of primers and unincorporated nucleotides from enzymatic reactions; separation of linearized from uncut vector and isolation of PCR products away from genomic DNA.

Specifications
SKU 111101200-CF
Application Notes The GENECLEAN® SPIN Kit is used to purify fragments of DNA from 200 bp to 300 kb from solutions and agarose gels. Examples of applications are desalting, elimination of proteins from enzymatic reactions, removal of primers and unincorporated nucleotides from enzymatic reactions, separation of linearized from uncut vector and isolation of PCR products away from genomic DNA.
Brand GENECLEAN®
Components Geneclean® SPIN GLASSMILK™ |GeneClean® SPIN new wash solution |GeneClean® SPIN elution solution |SPIN filter tubes |catch tubes
Flash Point 21 °C/69.8 °F (Lit.)
Format Geneclean® slurry plus SPIN filter
Applications Examples of applications are desalting; elimination of proteins from enzymatic reactions; removal of primers and unincorporated nucleotides from enzymatic reactions; separation of linearized from uncut vector and isolation of PCR products away from genomic DNA.
Kit or Assay Type DNA purification kit
Materials Required Not Supplied 100% ethanol |Distilled water |Microcentrifuge |Vortexer |Water bath or heat block |1.5 ml microcentrifuge tubes
Molecule Enriched DNA
Personal Protective Equipment Personal-protective-equipment: Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
pH Geneclean SPIN New Wash pH Range: 7.4 - 7.7 |GC Spin Elution Solution pH Range: 6.8 - 7.2
Physical Appearance Solution |Slurry | Spin filter module
Preparation Method Before first use, add the 25 mL of 200 proof (100% ethanol) to the GENECLEAN® SPIN NEW Wash Concentrate (25 mL) and mix well. Do not use denatured alcohol because it can cause precipitation of salts. Label the container. Store tightly capped at 15°-30°C.
Presentation Kit
Product Families Description The GENECLEAN® SPIN kit simplifies the process of purifying DNA into three easy steps: Bind,Wash and Elute. Ethanol precipitation is never required and purified DNA is immediately ready for a wide variety of downstream applications.
Product Overview The GENECLEAN® SPIN kit includes SPIN filters. The use of SPIN filters prevents silica particle carry-over into cleaned DNA. Glassmilk™ slurry allows flexibility of reaction size and volume.
Recommended Replacement For 111102200
Research Areas Molecular Biology |Plant Biology |Cell Biology |Biochemistry |Genomics |Environmental and Ecology
Safety Symbol GHS02, GHS05, GHS06
Sample Type Agarose gels| solutions| enzymatic reactions
Sample Volume Up to ~300 mg gel slice or up to ~300 µl DNA solution
Storage and Handling Store at Room Temperature (15-30 °C).
Unit Definition Each prep uses 400 μl of GENECLEAN® SPIN GLASSMILK®, which will bind up to 5 μg of either ssDNA or dsDNA.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.