GENECLEAN III KIT--N/H

GENECLEAN III KIT--N/H

Synonyms DNA Clean-Up Kit

SKU 111001600
Price:
$347.95
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Description

Product Description

The GENECLEAN® III kit simplifies the process of purifying DNA into three easy steps: Bind,Wash and Elute. Ethanol precipitation is never required and purified DNA is immediately ready for a wide variety of downstream applications.

Application Notes

The GENECLEAN® III Kit is used to purify fragments of DNA from 200 bp to 20 kb from solutions and agarose gels. Examples of applications are desalting, elimination of proteins from enzymatic reactions, removal of primers and unincorporated nucleotides from enzymatic reactions, separation of linearized from uncut vector and isolation of PCR products away from genomic DNA.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Purify DNA from agarose gel| Desalting| Elimination of proteins from enzymatic reactions| Removal of primers and unincorporated nucleotides from enzymatic reactions| Separation of linearized from uncut vector| Isolation of PCR products away from genomic DNA

Specifications
SKU 111001600
Alternate Names DNA Clean-Up Kit
Application Notes The GENECLEAN® III Kit is used to purify fragments of DNA from 200 bp to 20 kb from solutions and agarose gels. Examples of applications are desalting, elimination of proteins from enzymatic reactions, removal of primers and unincorporated nucleotides from enzymatic reactions, separation of linearized from uncut vector and isolation of PCR products away from genomic DNA.
Brand GENECLEAN®
Components NaI| New wash concentrate| TBE modifier| EZ-Glassmilk™| Label Block| Elution Solution| BioFloat™ floating incubation rack
Format Geneclean® slurry
Applications Purify DNA from agarose gel| Desalting| Elimination of proteins from enzymatic reactions| Removal of primers and unincorporated nucleotides from enzymatic reactions| Separation of linearized from uncut vector| Isolation of PCR products away from genomic DNA
Kit or Assay Type DNA purification kit
Molecule Enriched DNA
Personal Protective Equipment Personal-protective-equipment: Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
pH Above 500 bp: 4.5 M NaI pH 7.0 - 7.4| Between 200 - 500 bp: 4.5 M NaI pH 6.0| NEW Wash: pH Range: 7.4 - 8.0| TBE Modifier: pH 5.8 ± 0.2
Physical Appearance Solution| Slurry
Preparation Method Pour contents of NEW Wash Concentrate (14 ml) into a bottle that accommodates at least 650 ml of liquid. Add 280 ml of distilled water and mix well. Add 310 ml of 100% (200 proof) ethanol and mix well. Label the prepared NEW Wash bottle with the label provided. Shake Glassmilk™ bottle until silica particles are in a uniform suspension.
Presentation Kit
Product Overview GeneClean® III Kit allows recovery of labelled DNA fragments with ease. EZ-Glassmilk™ slurry allows flexibility of reaction size and volume. EZ-Glassmilk™ is easier to suspend than original Glassmilk™.
Recommended Alternate GENECLEAN® SPIN Kit
Recommended Replacement For 111101200 | 111101400 | 111101600
Research Areas Molecular Biology |Plant Biology |Cell Biology |Biochemistry |Genomics |Environmental and Ecology
Safety Symbol GHS05, GHS06
Sample Volume Up to ~0.5 ml gel slice by volume or ~1.0 ml solution
Storage and Handling Store at Room Temperature (15-30 °C). NaI solution must be kept away from light and open air sources to prevent oxidation. Keep prepared New Wash Solution tightly capped to prevent evaporation of EtOH. Take care to keep silica particles out of the threads of the bottle and cap as this can cause an incomplete seal and result in evaporation of the solution in the Glassmilk slurry.
Trade Name GENECLEAN®
Unit Definition One µL of EZ-GLASSMILK™ will bind with one µg of DNA in 4.5 M Nal in 5 minutes at room temperature.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.