GENECLEAN II KIT--N/H

GENECLEAN II KIT--N/H

Synonyms DNA Clean-Up Kit

SKU 111001400
Price:
$238.10
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Description

Product Description

The GENECLEAN® II Kit is designed for the rapid purification of linear and plasmid DNA fragments from standard or low-melting agarose gels. This kit can also be used to purify DNA from polyacrylamide gels. Typical recovery of DNA is up to 80%. Each column can bind up to 10 μg of DNA from up to a 3.5 g agarose slice. The GENECLEAN® II Kit combines silica-binding technology with the convenience of a spin or vacuum column format. DNA fragments of interest are extracted from slices of an agarose gel and are bound to a silica membrane. Contaminants are removed by a simple spin or vacuum wash. The bound DNA is then eluted. DNA fragments of interest are extracted by solubilizing slices of an agarose gel. The Gel Solubilization Solution dissolves an agarose gel slice from gels run in TBE or TAE buffer. The extracted DNA fragments selectively adsorb onto the silica membrane in the presence of the Gel Solubilization Solution. Contaminants are removed by a simple spin or vacuum wash. Finally, the bound DNA is eluted in Tris buffer.

Application Notes

The GENECLEAN® II Kit is designed to optimally purify 200 bp to 20 kb DNA from TAE and TBE buffered agarose gels and solutions. The use of optional SPIN Filters allows isolation of fragments up to 300 kb. Glassmilk™ slurry allows flexibility of reacion size and volume. The purified DNA is suitable for a variety of downstream applications, such as sequencing, PCR, restriction digestion, cloning and labeling.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Purify DNA from agarose gel| Desalting| Elimination of proteins from enzymatic reactions| Removal of primers and unincorporated nucleotides from enzymatic reactions| Separation of linearized from uncut vector| Isolation of PCR products away from genomic DNA

Specifications
SKU 111001400
Alternate Names DNA Clean-Up Kit
Application Notes The GENECLEAN® II Kit is designed to optimally purify 200 bp to 20 kb DNA from TAE and TBE buffered agarose gels and solutions. The use of optional SPIN Filters allows isolation of fragments up to 300 kb. Glassmilk™ slurry allows flexibility of reacion size and volume. The purified DNA is suitable for a variety of downstream applications, such as sequencing, PCR, restriction digestion, cloning and labeling.
Brand GENECLEAN®
Components NaI| New Wash Concentrate| TBE Modifier| Glassmilk™| BioFloat™ floating incubation rack
Format Geneclean® slurry
Applications Purify DNA from agarose gel| Desalting| Elimination of proteins from enzymatic reactions| Removal of primers and unincorporated nucleotides from enzymatic reactions| Separation of linearized from uncut vector| Isolation of PCR products away from genomic DNA
Kit or Assay Type DNA purification kit
Molecule Enriched DNA
Personal Protective Equipment Personal-protective-equipment: Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
pH NAI pH Range: 7.0-7.4| Geneclean New Wash pH Range: 7.4 - 8.0| TBE Modifier pH Range: 5.8 ± 0.2
Physical Appearance Solution| Slurry
Preparation Method Pour contents of NEW Wash Concentrate (14 ml) into a bottle that accommodates at least 650 ml of liquid. Add 280 ml of distilled water and mix well. Add 310 ml of 100% (200 proof) ethanol and mix well. Label the prepared NEW Wash bottle with the label provided. Shake Glassmilk™ bottle until silica particles are in a uniform suspension.
Recommended Alternate GENECLEAN® Kit| GENECLEAN® III Kit| GENECLEAN® SPIN Kit| GENECLEAN® Turbo Kit
Research Areas Molecular Biology |Plant Biology |Cell Biology |Biochemistry |Genomics |Environmental and Ecology
Safety Symbol GHS05, GHS06
Sample Type TAE or TBE buffered agarose gels| solutions| enzymatic reactions
Sample Volume Up to ~0.5 ml gel slice by volume or ~1.0 ml solution
Storage and Handling Store at Room Temperature (15-30 °C). NaI solution must be kept away from light and open air sources to prevent oxidation. Keep prepared New Wash Solution tightly capped to prevent evaporation of EtOH. Take care to keep silica particles out of the threads of the bottle and cap as this can cause an incomplete seal and result in evaporation of the solution in the Glassmilk slurry.
Trade Name GENECLEAN®
Unit Definition 1 μl of GLASSMILK® will bind 1-2 μg of DNA in 4.5 M Nal in 5 minutes at room temperature.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.