Dino

GENECLEAN KIT--N/H

GENECLEAN KIT--N/H

Synonyms DNA Clean-Up Kit

Packaging size: 200 Preps

SKU 111001200
Price:
$196.30
In Stock
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Description

Product Description

The GENECLEAN® kit simplifies the process of purifying DNA into three easy steps: Bind,Wash and Elute. Ethanol precipitation is never required and purified DNA is immediately ready for a wide variety of downstream applications. The GENECLEAN® Kit is used to purify fragments of DNA from 200 bp to 20 kb. The use of optional SPIN Filters allows isolation of fragments up to 300 kb.

Application Notes

The GENECLEAN® kit simplifies the process of purifying DNA into three easy steps: Bind,Wash and Elute. Ethanol precipitation is never required and purified DNA is immediately ready for a wide variety of downstream applications. The GENECLEAN® Kit is used to purify fragments of DNA from 200 bp to 20 kb. The use of optional SPIN Filters allows isolation of fragments up to 300 kb.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Purify DNA from agarose gel| Desalting| Elimination of proteins from enzymatic reactions| Removal of primers and unincorporated nucleotides from enzymatic reactions| Separation of linearized from uncut vector| Isolation of PCR products away from genomic DNA

Specifications
SKU 111001200
Application Notes The GENECLEAN® kit simplifies the process of purifying DNA into three easy steps: Bind,Wash and Elute. Ethanol precipitation is never required and purified DNA is immediately ready for a wide variety of downstream applications. The GENECLEAN® Kit is used to purify fragments of DNA from 200 bp to 20 kb. The use of optional SPIN Filters allows isolation of fragments up to 300 kb.
Brand GENECLEAN®
Components NaI| New Wash Concentrate| Glassmilk™| BioFloat™ floating incubation rack
Format Geneclean® slurry
Applications Purify DNA from agarose gel| Desalting| Elimination of proteins from enzymatic reactions| Removal of primers and unincorporated nucleotides from enzymatic reactions| Separation of linearized from uncut vector| Isolation of PCR products away from genomic DNA
Kit or Assay Type DNA purification kit
Materials Required Not Supplied 1.5 ml microcentrifuge tubes, Clean bottle that accommodates up to 350 ml, 100% ethanol, TE buffer, Distilled water, Microcentrifuge, Heated water bath, Vortexer
Molecule Enriched DNA
Pack Size 200 Preps
Personal Protective Equipment Personal-protective-equipment: Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
pH NAI pH Range: 7.0-7.4| Geneclean New Wash pH Range: 7.4 - 7.7
Physical Appearance Solution| Slurry
Preparation Method Pour contents of NEW Wash Concentrate (14 ml) into a bottle that accommodates at least 650 ml of liquid. Add 280 ml of distilled water and mix well. Add 310 ml of 100% (200 proof) ethanol and mix well. Label the prepared NEW Wash bottle with the label provided. Shake Glassmilk™ bottle until silica particles are in a uniform suspension.
Presentation Kit
Product Overview The GENECLEAN® Kit includes Glassmilk™ slurry that allows flexibility of reaction size and volume. It also contains sodium iodide solutions for disolving gels and the NEW Wash Concentrate.
Recommended Alternate GENECLEAN® II Kit| GENECLEAN® III Kit| GENECLEAN® SPIN Kit| GENECLEAN® Turbo Kit
Research Areas Molecular Biology |Plant Biology |Cell Biology |Biochemistry |Genomics |Environmental and Ecology
Safety Symbol GHS05, GHS06
Sample Type TAE buffered agarose gels| solutions| enzymatic reactions
Sample Volume Up to ~0.5 ml gel slice by volume or ~1.0 ml solution
Storage and Handling Store at Room Temperature (15-30 °C). NaI solution must be kept away from light and open air sources to prevent oxidation. Keep prepared New Wash Solution tightly capped to prevent evaporation of EtOH. Take care to keep silica particles out of the threads of the bottle and cap as this can cause an incomplete seal and result in evaporation of the solution in the Glassmilk slurry.
Unit Definition 1 μl of GLASSMILK™ will bind 1-2 μg of DNA in 4.5 M Nal in 5 minutes at room temperature.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.