Pluronic® F-68 polyol, 10% solution, (100X), 100 mL

Pluronic F-68® is a difunctional block copolymer surfactant terminating in primary hydroxyl groups. It is a non-ionic detergent that protects cells from hydrodynamic damage. It can be also used for in vitro isolation of lymphocytes from diluted whole blood.

Application Notes

Pluronic® F-68 is a widely used non-ionic surfactant in cell culture, offering protection against shear stress in suspension cultures during stirring, transfer, freezing, and thawing. It also helps reduce foaming in stirred cultures and minimizes cell attachment to glass surfaces, preventing agglomeration.

Optimized Concentration for Different Applications

• Common Working Concentration: 0.1% (1 g/L) – Balances shear protection and cell growth.
• Typical Range: 0.01% to 0.2% – Depends on final application.
• Cryopreservation: Higher concentrations (0.2%) improve cell viability by reducing ice crystal formation and osmotic stress.
• Lymphocyte Isolation: Used for in vitro isolation of lymphocytes from diluted whole blood.

Pluronic® F-68 is essential in bioprocessing, gene therapy, vaccine production, and cryopreservation, ensuring optimal cell growth, viability, and stability in various applications.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Mammalian Cell Culture | Cell Culture