10X Dulbecco's Phosphate Buffered Saline, Without Calcium and Magnesium

10X Dulbecco's Phosphate Buffered Saline, Without Calcium and Magnesium

Synonyms DPBS

Packaging size: 500 mL

SKU 091960454
Price:
$29.95
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Description

Product Description

Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions: 1. Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance; 2. Provides cells with water and certain bulk inorganic ions essential for normal cell metabolism; 3. Combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism; 4. Provides a buffering system to maintain the medium within the physiological pH range (7.2 - 7.6)

Application Notes

Dulbecco's Phosphate Buffered Saline 10X is a stock solution used to prepare 1X D-PBS in cell culture grade water. D-PBS is used to wash cells during preparation and serial transfer Phosphate buffering maintains the pH in the physiological range. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells. Used to wash cells during preparation and serial transfer.

Key Applications

Mammalian Cell Culture

Specifications
SKU 091960454
Alternate Names DPBS
Application Notes Dulbecco's Phosphate Buffered Saline 10X is a stock solution used to prepare 1X D-PBS in cell culture grade water. D-PBS is used to wash cells during preparation and serial transfer Phosphate buffering maintains the pH in the physiological range. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells. Used to wash cells during preparation and serial transfer.
Concentration 10X
Formulation Liquid | (as 10X) Inorganic Salts: Potassium Chloride [KCl]: 2000.00 mg/L; Potassium Phosphate Monobasic [KH<sub>2</sub>PO<sub>4</sub>]: 2000.00 mg/L; Sodium Chloride [NaCl]: 80000.00 mg/L; Sodium Phosphate Dibasic [Na<sub>2</sub>HPO<sub>4</sub>]: 11500.00 mg/L
Applications Mammalian Cell Culture
Pack Size 500 mL
pH 6.5 - 7.5
Physical Appearance Clear; Colorless Solution
Sterility Sterile
Storage and Handling Store at Room Temperature (15-30°C).