10X Dulbecco's Phosphate Buffered Saline, Without Calcium and Magnesium
Product Description
Role of a balanced salt solution in cell culture is multifaceted and can be divided into four principal functions: 1. Serves as an irrigating, transporting and diluting fluid while maintaining intra- and extracellular osmotic balance; 2. Provides cells with water and certain bulk inorganic ions essential for normal cell metabolism; 3. Combined with a carbohydrate, such as glucose, provides the principal energy source for cell metabolism; 4. Provides a buffering system to maintain the medium within the physiological pH range (7.2 - 7.6)
Application Notes
Dulbecco's Phosphate Buffered Saline 10X is a stock solution used to prepare 1X D-PBS in cell culture grade water. D-PBS is used to wash cells during preparation and serial transfer Phosphate buffering maintains the pH in the physiological range. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells. Used to wash cells during preparation and serial transfer.
Key Applications
Mammalian Cell Culture
| SKU | 091960454 |
| Application Notes | Dulbecco's Phosphate Buffered Saline 10X is a stock solution used to prepare 1X D-PBS in cell culture grade water. D-PBS is used to wash cells during preparation and serial transfer Phosphate buffering maintains the pH in the physiological range. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells. Used to wash cells during preparation and serial transfer. |
| Concentration | 10X |
| Formulation Details | Liquid | (as 10X) Inorganic Salts: Potassium Chloride [KCl]: 2000.00 mg/L; Potassium Phosphate Monobasic [KH<sub>2</sub>PO<sub>4</sub>]: 2000.00 mg/L; Sodium Chloride [NaCl]: 80000.00 mg/L; Sodium Phosphate Dibasic [Na<sub>2</sub>HPO<sub>4</sub>]: 11500.00 mg/L |
| Applications | Mammalian Cell Culture |
| Pack Size | 500 mL |
| pH | 6.5 - 7.5 |
| Physical Appearance | Clear; Colorless Solution |
| Sterility | Sterile |
| Storage and Handling | Store at Room Temperature (15-30°C). |