Trypan Blue, 0.4% Solution In PBS

Trypan Blue, 0.4% Solution In PBS

Synonyms Direct Blue 14; Niagara Blue 3B; Chlorazol blue 3B; Benzo blue 3B; Dianil blue H3G; Congo blue 3B; Naphthamine blue 3BX; Benzamine blue 3B; Azidine blue 3B; Diamine Blue 3B;

CAS Number: 72-57-1 Molecular Formula: C34H24N6Na4O14S4 Molecular Weight: 960.82

Packaging size: 100 mL

SKU 091691049
Price:
$17.95
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Description

Product Description

Trypan Blue is a blue acid dye with a strong affinity for cellulose containing substrates such as cotton; less affinity for proteinaceous materials. Trypan blue solution may be used in trypan blue based cytotoxitiy and proliferation assays. Trypan Blue is used as a vital dye which is especially important because it is taken up by the reticuloendothelial system. Clark describes assays for the study of teratogenic action of trypan blue on embryonic tissues using Davis and Sauter's fluorescence method and for the staining of collagen, including very fine fibrils, muscle and cornified epithelium using the Van Gieson method. Trypan blue is also recommended for use in dye exclusion procedures for viable cell counting. Non-viable cells will up-take trypan blue at a faster rate than viable cells.

Application Notes

Trypan Blue is used as a vital dye which is especially important because it is taken up by the reticuloendothelial system. Clark describes assays for the study of teratogenic action of trypan blue on embryonic tissues using Davis and Sauter's fluorescence method and for the staining of collagen, including very fine fibrils, muscle and cornified epithelium using the Van Gieson method. Trypan blue is also recommended for use in dye exclusion procedures for viable cell counting. Non-viable cells will up-take trypan blue at a faster rate than viable cells.

Key Applications

Cell Counting; Cell Viability; Cryopreservation | Cytotoxitiy and proliferation assays

Specifications
SKU 091691049
Alternate Names Direct Blue 14; Niagara Blue 3B; Chlorazol blue 3B; Benzo blue 3B; Dianil blue H3G; Congo blue 3B; Naphthamine blue 3BX; Benzamine blue 3B; Azidine blue 3B; Diamine Blue 3B;
Application Notes Trypan Blue is used as a vital dye which is especially important because it is taken up by the reticuloendothelial system. Clark describes assays for the study of teratogenic action of trypan blue on embryonic tissues using Davis and Sauter's fluorescence method and for the staining of collagen, including very fine fibrils, muscle and cornified epithelium using the Van Gieson method. Trypan blue is also recommended for use in dye exclusion procedures for viable cell counting. Non-viable cells will up-take trypan blue at a faster rate than viable cells.
Concentration 0.4% (w/v) Solution
Formulation Liquid | Potassium Phosphate Dibasic: 600.00000 mg/L; Sodium Chloride [NaCl]: 8100.00000 mg/L; Trypan Blue: 4000.00000 mg/L
Applications Cell Counting; Cell Viability; Cryopreservation | Cytotoxitiy and proliferation assays
Molecular Formula C<sub>34</sub>H<sub>24</sub>N<sub>6</sub>Na<sub>4</sub>O<sub>14</sub>S<sub>4</sub>
Molecular Weight 960.82
Pack Size 100 mL
pH 7.0 - 8.0
Physical Appearance Dark Blue Solution
Presentation Potassium Phosphate Dibasic: 600.00000 mg/L; Sodium Chloride [NaCl]: 8100.00000 mg/L; Trypan Blue: 4000.00000 mg/L | Solution
Solubility Soluble in ethylene glycol monomethyl ether (methyl cellosolve; EGME) (20 mg/mL); Slightly soluble in water or ethanol (0.6 mg/mL); almost insoluble in alcohol.
Storage and Handling Store at Room Temperature (15-30°C).