Phenol red is an acid-base indicator. It is made by condensing two moles of phenol with one mole of o-sulfobenzoic acid anhydride. Phenol red in tissue culture media can act as a weak estrogen, especially with human breast cancer cells. Phenol red can be used to measure hydrogen peroxide in cultured macrophages in multiwell plates.
Application Notes
Phenol Red is used as a pH indicator in cell culture applications. A solution of phenol red will have a yellow color at a pH of 6.4 or below and a red color at a pH of 8.2 and above. Phenol red in tissue culture media can act as a weak estrogen, especially with human breast cancer cells. Phenol red can be used to measure hydrogen peroxide in cultured macrophages in multiwell plates.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Mammalian Cell Culture | pH indicator in cell culture applications
| SKU | 091690049 |
| Alternate Names | Phenolsulfonphthalein |
| Application Notes | Phenol Red is used as a pH indicator in cell culture applications. A solution of phenol red will have a yellow color at a pH of 6.4 or below and a red color at a pH of 8.2 and above. Phenol red in tissue culture media can act as a weak estrogen, especially with human breast cancer cells. Phenol red can be used to measure hydrogen peroxide in cultured macrophages in multiwell plates. |
| Base Catalog Number | 1690049 |
| CAS # | 34487-61-1 |
| Format | Solution |
| Formulation Details | Liquid | Phenol Red Sodium Salt: 5000.00000 mg/L |
| Molecular Formula | C19H13O5SNa(Lit.) |
| Molecular Weight | 376.36(Lit.) |
| Pack Size | 100 mL |
| pH | Visual transition interval is from pH 6.8 (yellow) to pH 8.2 (red) |
| Solubility | Soluble in water (100 mg/mL-clear red solution) methanol or ethanol (200 mg/mL). |
| Sterility | Sterile |
| Typical Working Concentration | It is typically used in cell culture media at 11 mg/L. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
![MP COA [091690049] lot ID: FC0842](https://www.mpbio.com/static/version1742546773/frontend/Mpbio/default/en_US/images/document.png "MP COA [091690049] lot ID: FC0842")