10X Trypsin HBSS Without Calcium and Magnesium

10X Trypsin HBSS Without Calcium and Magnesium

Synonyms Trypsin 1:250; Trypsin-EDTA solution

CAS Number: 9002-07-7 (trypsin) Molecular Weight: Approximately 23;000 or 22000 (for human)

Packaging size: 100 mL

SKU 091689349
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Description

Product Description

Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture.

Application Notes

Trypsin-EDTA solutions are used to release adherent cells from culture surfaces. They are generally composed of variable concentrations of natural porcine pancreas-derived trypsin and EDTA. Trypsin 1X solutions can range from 0.025% to 0.5%. The reasons for the range of concentrations are differences in required trypsin activity or potency; differences in incubation times; and cell line susceptibility. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Various formulations should be tested to determine the best product for a specific application.

Key Applications

Cell Counting | Cell Cell Viability | Cryopreservation | Cell dissociation

Specifications
SKU 091689349
Alternate Names Trypsin 1:250; Trypsin-EDTA solution
Application Notes Trypsin-EDTA solutions are used to release adherent cells from culture surfaces. They are generally composed of variable concentrations of natural porcine pancreas-derived trypsin and EDTA. Trypsin 1X solutions can range from 0.025% to 0.5%. The reasons for the range of concentrations are differences in required trypsin activity or potency; differences in incubation times; and cell line susceptibility. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Various formulations should be tested to determine the best product for a specific application.
Concentration 10X | 2.5% (w/v) solution
Formulation Details Liquid | Potassium Chloride [KCl]: 400.0000 mg/L; Potassium Phosphate Monobasic [KH<sub>2</sub>PO<sub>4</sub>]: 60.0000 mg/L; Sodium Bicarbonate [NaHCO<sub>3</sub>]: 350.0000 mg/L; Sodium Chloride [NaCl]: 8000.0000 mg/L; Sodium Phosphate Dibasic [Na<sub>2</sub>HPO<sub>4</sub>]: 47.5000 mg/L; Dextrose: 1000.0000 mg/L; Phenol Red Sodium Salt: 17.0000 mg/L; Trypsin 1:250: 2500.0000 mg/L
Applications Cell Counting | Cell Cell Viability | Cryopreservation | Cell dissociation
Molecular Weight Approximately 23;000 or 22000 (for human)
Pack Size 100 mL
pH 5.0 - 6.0
Physical Appearance Clear; Colorless Solution
Source Porcine (for trypsin)
Specific Activity One USP trypsin unit is the activity causing a change in absorbance of 0.003 per minute under the conditions specified.
Specificity The protease activity of trypsin is highly specific toward positively charged side chains with lysine and arginine. It forms complexes with α2-macroglobulin and can be used in the isolation of intact; detergent-free phycobilisomes and in the hydrolysis/condensation of carboxylic ester bonds.
Sterility Sterile
Storage and Handling Store between 0 and -20°C.