RPMI 1640 With 2 g/L Sodium Bicarbonate, Without L-Glutamine & Glucose

RPMI 1640 With 2 g/L Sodium Bicarbonate, Without L-Glutamine & Glucose

Synonyms 1X Liquid

Packaging size: 500 mL

SKU 091646854
Price:
$34.20
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Description

Product Description

RPMI 1640 Medium was originally developed by Moore and his colleagues at Roswell Park Memorial Institute (RPMI). It was based on the RPMI 1630 line of media which utilized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. A Dutch Modification of RPMI 1640 is also available through MP. The Dutch Modification contains 6400 mg/L sodium chloride instead of 6000 mg/L; contains 1000 mg/L sodium bicarbonate instead of 2000 mg/L; and contains 20 mM HEPES.

Application Notes

RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. RPMI has successfully been used for the cultivation of normal human and neoplastic leukocytes. It is now a popular general purpose medium when properly supplemented.

Key Applications

Mammalian Cell Culture | Culture human leukemic cells and a variety of mammalian cells

Specifications
SKU 091646854
Alternate Names 1X Liquid
Application Notes RPMI 1640 Medium was originally developed to culture human leukemic cells in suspension and as a monolayer. Roswell Park Memorial Institute (RPMI) 1640 Medium has since been found suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas. RPMI has successfully been used for the cultivation of normal human and neoplastic leukocytes. It is now a popular general purpose medium when properly supplemented.
Formulation Details Liquid
Applications Mammalian Cell Culture | Culture human leukemic cells and a variety of mammalian cells
Pack Size 500 mL
pH 6.8 - 7.7
Physical Appearance Clear; Red Solution
Presentation Solution
Product Overview RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5–10% CO<sub>2</sub> environment to maintain physiological pH.
Sterility Sterile | Sterile Filtered
Storage and Handling Store at 2-8°C.