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RPMI (1X) w/o L-glutamine, L-cysteine, L-cystine, and L-methionine, 500 mL

RPMI (1X) w/o L-glutamine, L-cysteine, L-cystine, and L-methionine, 500 mL

Synonyms 1X Liquid

Packaging size: 500 mL

SKU 091646454
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Description

Product Description

RPMI 1640 Medium was originally developed by Moore and his colleagues at Roswell Park Memorial Institute (RPMI). It was based on the RPMI 1630 line of media which utilized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. A Dutch Modification of RPMI 1640 is also available through MP. The Dutch Modification contains 6400 mg/L sodium chloride instead of 6000 mg/L; contains 1000 mg/L sodium bicarbonate instead of 2000 mg/L; and contains 20 mM HEPES.

Application Notes

RPMI has successfully been used for the cultivation of normal human and neoplastic leukocytes. It is now a popular general purpose medium when properly supplemented. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells.

Key Applications

Mammalian Cell Culture

Specifications
SKU 091646454
Alternate Names 1X Liquid
Application Notes RPMI has successfully been used for the cultivation of normal human and neoplastic leukocytes. It is now a popular general purpose medium when properly supplemented. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells.
Base Catalog Number 1646454
Concentration 1X
Format Solution
Formulation Details Liquid
Media Type RPMI
Pack Size 500 mL
pH 6.8-7.4
Sterility Sterile | Sterile Filtered