MEM Amino Acids (50X Solution) Without L-Glutamine
In many cell culture applications using defined media for the in vitro cultivation of cells, the addition of supplemental nutrients and reagents is required. Additives such as antibiotics, buffers, and stains are frequently used to prevent bacterial contamination, control pH, and visibly monitor media conditions. Other supplements such as amino acids and vitamins are useful for enriched media beyond their normal concentrations. Attachment factors and transport factors are added to growth media to facilitate rapid cell propagation and enhance proper cell metabolism. Still other media supplements may be added to create conditions which will elicit specific cellular responses and effects, such as altered protein biosynthesis, accelerated antibody production and secretion, toxic response mechanisms, etc. As a result, the availability of a wide variety of media supplements is desirable when designing cell culture experiments.
Adding supplements of amino acids to media both stimulates growth and prolongs the viability of cells in culture. This concentrated supplement adds nutrients and reduces the biosynthetic burden on cell in vitro.
Mammalian Cell Culture | Stimulates growth and prolongs the viability of cells in culture
|Alternate Names||MEM Amino Acids (50x) solution|
|Application Notes||Adding supplements of amino acids to media both stimulates growth and prolongs the viability of cells in culture. This concentrated supplement adds nutrients and reduces the biosynthetic burden on cell in vitro.|
|Formulation Details||Liquid | Amino Acids: L-Arginine HCl 6320 mg/L; L-Cystine 1201 mg/L; L-Histidine HCl H<sub>2</sub>O 2095 mg/L; L-Isoleucine 2625 mg/L; L-Leucine 2625 mg/L; L-Lysine HCl 3653 mg/L; L-Methionine 745 mg/L; L-Phenylalanine 1651 mg/L; L-Threonine 2382 mg/L; L-Tryptophan 510 mg/L; L-Tyrosine 1811 mg/L; L-Valine 2345 mg/L.|
|Applications||Mammalian Cell Culture | Stimulates growth and prolongs the viability of cells in culture|
|Pack Size||100 mL|
|pH||9.6 - 10.0|
|Physical Appearance||Clear; Pale Yellow Solution|
|Product Families Description||<b>50X MEM Concentrate, Modified</b><br>Without L-glutamine|
|Storage and Handling||Store at +4°C.|