1X Mccoy 5A Medium, Iwakata & Grace Modification With L-Glutamine
McCoy's 5A medium, as modified by Iwakata and Grace, is identical to RPMI 1629. Originally in 1959, McCoy and his colleagues described the amino acid requirements for the in vitro culturing of Novikoff Hepatoma cells. Basal Medium 5A was modified to create what is known as McCoy's 5A Medium for these investigations. Hsu and Kellogg further demonstrated the use of this medium to support the growth of primary cultures derived from normal bone marrow, testes, mouse kidney, skin, gingiva, rat embryo and other tissues. MP's medium is additionally suited for the propagation of leukocytes, biopsy tissues and the most demanding primary and continuous cell types. It is also available as modified by Park and Terasaki. The Park and Terasaki Modification (PT) differs from the Iwakata and Grace Modication (IG) in that PT contains slightly modified levels of L-glutamic acid, glycine, L-hydroxyproline, L-proline, L-serine, L-threonine, L-tryptophan, and L-valine. The PT modification also contains the additions of penicillin, dihydrostreptomycin sulfate, gentamycin, fetal bovine serum and HEPES.
1× McCoy 5A Medium, Iwakata Grace Modification with L-Glutamine is excellent for the propagation of leukocytes, biopsy tissues, a broad range of human and rat normal and transformed cell types, and the most demanding primary and continuous cell lines.
Mammalian Cell Culture
|Application Notes||1× McCoy 5A Medium, Iwakata Grace Modification with L-Glutamine is excellent for the propagation of leukocytes, biopsy tissues, a broad range of human and rat normal and transformed cell types, and the most demanding primary and continuous cell lines.|
|Applications||Mammalian Cell Culture|
|Pack Size||500 mL|
|pH||6.6 - 7.2|
|Physical Appearance||Clear Red Solution|
|Product Overview||McCoy’s media were originally formulated for the growth and support of human lymphocytes. The 5A modification originated as a reformulation of McCoy’s by Hsu and Kellogg. It was then further modified by Iwakata and Grace featuring increased levels of i-inositol and glucose. This final modification produced a media identical to RPMI 1629. McCoy’s 5A media support the indefinite proliferation of Walker 256 carcinoma cells.|
|Storage and Handling||Store at +4°C; protect from light.|