Ham's F-12 (modified) wih L-glutamine, w/o sodium bicarbonate

Ham's F-12 (modified) wih L-glutamine, w/o sodium bicarbonate

SKU 09104212-CF
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Product Description

Originally, Ham's Nutrient Mixtures were developed for the clonal growth of several Chinese hamster ovary (CHO) clone cells, Hela cells and mouse L-cells. Both F-10 and F-12 are formulated for use with or without serum, depending on the type of cells being cultured. F-10 has demonstrated satisfactory growth of human diploid cells, white blood cells for chromosomal analysis and primary rat, rabbit and chicken tissue explants. Ham's F-12 is well suited for the growth of primary rat hepatocytes and prostate epithelial cells. It is also the medium of choice for toxicity assays using CHO cells. MP also offers Ham's F-12 supplemented with 25 mM HEPES for improved buffering in the optimum pH range of 7.2-7.4. F12K is a modification of Ham's F-12 and Coon's F-12 with increased amino acid and pyruvate concentrations. Additionally, the salts have been modifed (Konigsbergs). F12K has been developed for culturing differentiated rat and chicken cells, as well as, primary human liver cells. MgCl2 has been substituted by MgSO4 containing an equivalent amount of Mg ion.

Key Applications

Mammalian Cell Culture

SKU 09104212-CF
Base Catalog Number 1042120,1042122
Formulation Details With L-Glutamine; Without Sodium Bicarbonate
Physical Appearance Off-white Powder
Product Families Description (Powder)<br>With <span class="small-uc">L</span>-glutamine<br>Without sodium bicarbonate
Storage and Handling Store at 0-5°C.
Format solution
Media Type Ham's F12