Nutrient Broth

Nutrient Broth

Synonyms Standard - Nutrient Broth

Packaging size: 500 g

SKU 091007917
Price:
$97.25
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Description

Product Description

Nutrient Broth is a general purpose medium used for cultivating a broad variety of fastidious and non-fastidious microorganisms with non-exacting nutritional requirements. Peptone and yeast extract provide nitrogenous compounds, vitamin B complex, amino acids and other essential growth nutrients. Sodium chloride is used to maintain the osmotic balance and glucose is the fermentable carbohydrate source of the media.

Application Notes

Nutrient Broth is used in many laboratory procedures as is, or with added indicators, carbohydrates, organic liquids, salts, etc. This medium is used in accordance with official recommended procedures for the bacteriological analyses of water, milk and dairy products, in foods of important sanitation, tests for sensitivity and resistance and as a base to prepare media supplemented with other nutrients. It can be used for enumeration, isolation and enrichment as well as providing a high grade base for the preparation of special media. With the addition of blood, ascites fluid or serum, they can also be used to cultivate streptococci, pneumococci and erysipelas species etc.

Key Applications

Cell Culture | Cell growth supplement

Specifications
SKU 091007917
Alternate Names Standard - Nutrient Broth
Application Notes Nutrient Broth is used in many laboratory procedures as is, or with added indicators, carbohydrates, organic liquids, salts, etc. This medium is used in accordance with official recommended procedures for the bacteriological analyses of water, milk and dairy products, in foods of important sanitation, tests for sensitivity and resistance and as a base to prepare media supplemented with other nutrients. It can be used for enumeration, isolation and enrichment as well as providing a high grade base for the preparation of special media. With the addition of blood, ascites fluid or serum, they can also be used to cultivate streptococci, pneumococci and erysipelas species etc.
Formulation Details Powder | Nutrient Broth is a liquid medium; produced according to the formula from APHA and AOAC. Approximate formula: Gelatin Peptone: 5.0 g/L; Beef Extract: 3.0 g/L; Final pH: 6.9 +/- 0.1
Applications Cell Culture | Cell growth supplement
Pack Size 500 g
pH 6.7 - 7.1 (8 g/L)
Physical Appearance Tan Powder
Preparation Method Dissolve 8 g of the medium in a liter of deionized or distilled water. Dispense as desired and sterilize at 121 °C (15 lbs. of pressure) for 15 minutes.
Presentation Nutrient Broth is a liquid medium; produced according to the formula from APHA and AOAC. Approximate formula: Gelatin Peptone: 5.0 g/L; Beef Extract: 3.0 g/L; Final pH: 6.9 +/- 0.1 | Powder
Solubility Soluble in water (8 g/L).
Sterility Sterilize at 121 °C (15 lbs. of pressure) for 15 minutes.
Storage and Handling Store at Room Temperature (15-30°C)