Brain Heart Infusion Broth

Brain Heart Infusion Broth

Synonyms BHI Broth; Brain Heart Broth

Packaging size: 500 g

SKU 091002417
Price:
$89.60
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Description

Product Description

Brain Heart Infusion Broth is a modification of the media by Rosenow and Hayden. The infusion from calf brains has replaced the brain tissue and disodium phosphate has replaced the calcium carbonate buffer.

Application Notes

Brain Heart Infusion Broth is developed for the cultivation of a large variety of fastidious and non-fastidious, aerobic and anaerobic microorganisms including bacteria, yeast and fungi (molds). It is very useful for blood cultures. It can also be used to detect organisms in blood of patients on sulfonamide therapy by the addition of PABA (0.05 g/L).

Key Applications

Yeast Cell Culture

Specifications
SKU 091002417
Alternate Names BHI Broth; Brain Heart Broth
Application Notes Brain Heart Infusion Broth is developed for the cultivation of a large variety of fastidious and non-fastidious, aerobic and anaerobic microorganisms including bacteria, yeast and fungi (molds). It is very useful for blood cultures. It can also be used to detect organisms in blood of patients on sulfonamide therapy by the addition of PABA (0.05 g/L).
Formulation Details Powder | Beef Heart Infusion from Solids 17.5; Dextrose 2.0; Gelatin Peptone 10.0; Disodium Phosphate 2.5; Sodium Chloride 5.0 | Powder
Applications Yeast Cell Culture
Pack Size 500 g
pH 7.2 - 7.6
Physical Appearance Pale Yellow Powder
Preparation Method Suspend 37 g of the medium in one liter of deionized or distilled water and heat slowly, if necessary. The addition of a small amount of agar (0.1%) is recommended for the growth and isolation of pathogens from blood and other specimen material (anaerobic organisms). Dispense in tubes and sterilize at 121 °C (15lbs. of pressure) for 15 minutes.
Presentation Beef Heart Infusion from Solids 17.5; Dextrose 2.0; Gelatin Peptone 10.0; Disodium Phosphate 2.5; Sodium Chloride 5.0 | Powder
Solubility Soluble in 3.7% distilled or deionized water (clear, light to medium amber solution).
Storage and Handling Store at room temperature (15-30°C).