Monoclonal Mouse Anti-Epithelial Keratin - AE5

Monoclonal Mouse Anti-Epithelial Keratin - AE5

Synonyms Type II Cytokeratin

Packaging size: 200 µg

SKU 08691431
Price:
$468.85
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Description

Product Description

Monoclonal Mouse Anti-Epithelial Keratin antibody is specific for the basic 64K keratin which, together with the acidic 55K keratin, has been designated as a marker for "corneal-type differentiation".

Application Notes

Monoclonal Mouse Anti-Epithelial Keratin - AE5 is used in immunoblotting; Indirect Immunofluorescence; Tissue sections. This antibody is specific for the basic 64 kDa (number 3) keratin which, together with the acidic 55 kDa (number 12) keratin, has been designated as a marker for "corneal-type differentiation." It also reacts with lip and snout (cow and rabbit) epithelia. For this antibody, unfixed, frozen sections perform better than formalin-fixed sections.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Key Applications

Immunoassays | Immunoblot | Immunofluorescence assays (IFA) | Immunohistochemistry (Paraffin)

Specifications
SKU 08691431
Alternate Names Type II Cytokeratin
Antibody Category Monoclonal
Antibody Type Monoclonal Antibody
Application Notes Monoclonal Mouse Anti-Epithelial Keratin - AE5 is used in immunoblotting; Indirect Immunofluorescence; Tissue sections. This antibody is specific for the basic 64 kDa (number 3) keratin which, together with the acidic 55 kDa (number 12) keratin, has been designated as a marker for "corneal-type differentiation." It also reacts with lip and snout (cow and rabbit) epithelia. For this antibody, unfixed, frozen sections perform better than formalin-fixed sections.
Clone Name AE5
Concentration Antibody Conc.:1.0 mg/ml
Conjugate Unconjugated
Formulation Details Liquid
Gene Symbol and Aliases KRT8 KO K8 CYK8 Keratin-8 CK-8
Host Mouse
Isotype Purified Ig fraction from ascites
Applications Immunoassays | Immunoblot | Immunofluorescence assays (IFA) | Immunohistochemistry (Paraffin)
Pack Size 200 µg
Physical Appearance Liquid
Preparation Method The AE5 monoclonal anti-keratin was prepared against human epidermal keratin by hybridoma technique using P3 x 63 Ag8 myeloma cells. The AE5 was reactive with the 64K keratins in the cornea.
Presentation Liquid
Research Areas Cytoskeletal Signaling
Solubility Soluble in water
Storage and Handling Store frozen at or below -20°C.
Trade Name ImmunO™
Typical Working Concentration The dilutions below are suggested as staring points. The dilution range for western blotting may be as great as 1:1000 - 1:3000. 1:500 (Immunoblot - human epithelial cornea) 1:50 (Immunofluorescence - human epithelial cornea) It is recommended that each lab obtain their own optimum working dilution.
Usage Statement Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.