Mouse Anti-Phosphotyrosine, (Clone PY20)
Antibody is packaged in quantities of 100 µg at a concentration of 1 mg/ml in 20 mM sodium phosphate, 150 mM sodium chloride, pH 7.5. It is a highly purified immunoglobulin fraction.
Western Blots, immunoprecipitation, immunohistochemistry and immunocytochemistry procedures and ELISA
Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.
Immunohistochemistry | Immunocytochemistry |Immunoblot | Immunoprecipitation
|Antibody Type||Monoclonal Antibody|
|Application Notes||Western Blots, immunoprecipitation, immunohistochemistry and immunocytochemistry procedures and ELISA|
|Immunogen||Phosphotyrosine conjugated to carrier protein (PY-KLH) was used as immunogen.|
|Applications||Immunohistochemistry | Immunocytochemistry |Immunoblot | Immunoprecipitation|
|Pack Size||100 µg|
|Research Areas||Phosphorylation de-phosphorylation|
|Solubility||Soluble in water|
|Specificity||Reacts specifically and strongly to phosphotyrosine containing proteins. It will not recognize phosphothreonine or phosphoserine containing proteins. This antibody is not species specific. Note: This antibody is known to be inhibited by divalent cations (>1mM) and high salt concentrations (>0.2M).|
|Storage and Handling||Store at 0-5°C.|
|Typical Working Concentration||1:5,000 for Western Blot|
|Usage Statement||Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.|