Mouse Anti-Actin, Monoclonal (Clone C4)

Mouse Anti-Actin, Monoclonal (Clone C4)

Synonyms Monoclonal Anti-Actin

Configure Your Product:

SKU 0869100-CF
Price:
$227.90
In Stock
Request a quote
Description

Product Description

The monoclonal actin (clone C4) is a mouse IgG1 (gamma 1 heavy/kappa light chain) antibody. The monoclonal actin (clone C4) is a mouse IgG1 (gamma 1 heavy/kappa light chain) antibody. Chicken gizzard actin was used as the immunogen. Immunoblots demonstrate specificity directed towards all actins ranging from yeast, to flies, to mouse, to man. These are skeletal alpha-actin, cardiac alpha-actin, smooth muscle gamma actin, cytoplasmic beta-actin, cytoplasmic gamma actin as well as Dictyostelium discoideum and Physarum olycephalum actins. The epitope for the antibody lies between residues 18-40, a highly conserved region of the molecule. This antibody can also be used for cells cultivated on slides, for frozen tissue sections and as a loading control for western blots.

Application Notes

Actin Monoclonal Antibody was used for western blot analysis of Cos-7 cell lysates to ensure equal protein loading. Western blot analysis of MDCK cell lysates were performed using monoclonal anti-actin antibody as a primary antibody. Also It's used in Indirect ELISA, Immunofluorescence, Immunoblots and Immunohistochemistry.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Key Applications

ELISA | Immunohistochemistry | Immunoblot | Immunofluorescence assays (IFA)

Specifications
SKU 0869100-CF
Alternate Names Monoclonal Anti-Actin
Antibody Type Monoclonal Antibody
Application Notes Actin Monoclonal Antibody was used for western blot analysis of Cos-7 cell lysates to ensure equal protein loading. Western blot analysis of MDCK cell lysates were performed using monoclonal anti-actin antibody as a primary antibody. Also It's used in Indirect ELISA, Immunofluorescence, Immunoblots and Immunohistochemistry.
Clone Name C4
Conjugate Unconjugated
Formulation Liquid
Host Mouse
Isotype IgG1, kappa
Applications ELISA | Immunohistochemistry | Immunoblot | Immunofluorescence assays (IFA)
Personal Protective Equipment Eyeshields, Gloves, half-mask respirator , multi-purpose combination respirator cartridge
Physical Appearance Liquid
Presentation Liquid
Recommended Replacement For 869139
Research Areas Cell Structure
Solubility Soluble in water
Specificity Immunoblots demonstrate specificity directed towards all six known vertebrate isoactins as well as Dictyostelium discoideum and Physarum polycephalum actins
Storage and Handling Store at -20°C. Storage at -70°C will give a longer storage time. Stable for weeks at 2-8°C. Avoid repeated freeze/thaw cycles.
Trade Name ImmunO™
Typical Working Concentration Indirect Immunofluorescence: 1:500 Immunoblots: 1:500 for DAB/peroxidase detection, actin band migrates at 42 kDa on SDS-PAGE. Immunohistochemistry: 1:500
Usage Statement Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.