Dog Anti-Sheep Red Blood Cells
Form: lyophilized antiserum
Applications: Positive Coombs' Test Control
Because anti-globulin positive canine red blood cells are unstable, MP offers dog antiserum to sheep red blood cells. This reagent may be used to sensitize fresh sheep red blood cells for use as a positive control. It may also be used to prepare red blood cells for the canine Rose-Waller procedure.
Dog anti-sheep red blood cells can be used to sensitize sheep red blood cells (RBCs) for use in either a canine Rose-Waaler test, or in canine antiglobulin test as a positive control.
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
|Antibody Type||Polyclonal Antibody|
|Application Notes||Dog anti-sheep red blood cells can be used to sensitize sheep red blood cells (RBCs) for use in either a canine Rose-Waaler test, or in canine antiglobulin test as a positive control.|
|Pack Size||1 mL|
|Physical Appearance||Lyophilized powder|
|Research Areas||Autoimmune Diseases|
|Solubility||Soluble in water|
|Specificity||Antiserum to sheep red blood cells is produced by immunization of dogs with sheep RBCs. The harvested antiserum is incubated at 60°C for 30 minutes to inactivate complement. The antiserum is tested to determine its agglutination titer and the sub-agglutination titer for sensitization of sheep RBCs in canine antiglobulin or Rose-Waaler procedures. The optimum dilution for sensitization of sheep RBCs in these procedures will vary from one laboratory to another. A sub-agglutinating dose is calculated for each lot of antiserum (see below). Please note that with some sheep RBC preparations; the sub-agglutinating dose may be as high as 1:500 p 1:2;000; even though we my report a much lower titer with our RBC preparation.|
|Storage and Handling||Store at 2-8°C.|