Mouse, Anti-Beta-Galactosidase, Purified Monoclonal Antibody

Mouse, Anti-Beta-Galactosidase, Purified Monoclonal Antibody

MDL Number: MFCD00162155

Packaging size: 100 µg

SKU 08633651
Price:
$187.75
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Description

Product Description

Anti-Galactosidase, purified monoclonal antibody [subclass IgG2a(k)] was purified from ascites of a mouse hybridoma and recognizes E. coli β-galactosidase. Recognizes the native and denatured enzyme near the C-terminal end.

Application Notes

Monoclonal anti-β-galactosidase antibodies may be used in dot blot, western blot and immunocytochemistry. This antibody may also be used for immunochemical assays such as ELISA, immunoblot and immunohistochemistry by using the step-wise procedure or by the preparation of β-galactosidase anti-β galactosidase (BBAG) complex. Additionally, this product is suitable for the detection and purification of recombinant fusion proteins encoded by cloning vectors containing the β-galactosidase gene. Monoclonal anti-β-galactosidase antibody is also used for the immunoenzymatic staining of bone marrow and blood smears, and tissue sections.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Key Applications

Immunoassays | Dot Blot | Immunoblot | Immunocytochemistry | Immunocytochemistry | Immunoblot | Dot blot

Specifications
SKU 08633651
Antibody Type Monoclonal Antibody
Application Notes Monoclonal anti-β-galactosidase antibodies may be used in dot blot, western blot and immunocytochemistry. This antibody may also be used for immunochemical assays such as ELISA, immunoblot and immunohistochemistry by using the step-wise procedure or by the preparation of β-galactosidase anti-β galactosidase (BBAG) complex. Additionally, this product is suitable for the detection and purification of recombinant fusion proteins encoded by cloning vectors containing the β-galactosidase gene. Monoclonal anti-β-galactosidase antibody is also used for the immunoenzymatic staining of bone marrow and blood smears, and tissue sections.
Clone Name 6A-11
Concentration 2.2 mg/mL
Conjugate Unconjugated
Formulation Purified
Host Mouse
Immunogen Betagalactosidase from E. coli
Isotype IgG2a, kappa
Applications Immunoassays | Dot Blot | Immunoblot | Immunocytochemistry | Immunocytochemistry | Immunoblot | Dot blot
MDL # MFCD00162155
Pack Size 100 µg
Physical Appearance Liquid
Purity Immunoaffinity Chromatography 
Recommended Replacement For 863363
Solubility Soluble in water
Species Reactivity Bacteria
Specificity The monoclonal antibody recognizes E. coli b-Galactosidase. In a dot blot assay the antibody reacts with both native and denatured b-Galactosidase.
Storage and Handling Store at -20°C. Avoid multiple freeze/thaw cycles.
Trade Name ImmunO™
Typical Working Concentration Dilutions:Tissue Staining: 1:5 - 1:20 Immunoblotting: 1:10 - 1:50 Elisa/FIA: 1:40 - 1:200 Dot Blot: 1:5000 Immunoprecipitation: 25 mg/ml
Usage Statement Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.