: Product is goat affinity purified F(ab')2 fragments to human IgM (5Fc µ) and buffer salts. The affinity purified F(ab')2 fragment is prepared from the specific goat IgG fraction by limited digestion with pepsin and solid phase absorption. The resulting affinity purified F(ab')2 fragment is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 µm filter, and vialed.
Application Notes
This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Immunoassays
SKU | 0856960 |
Antibody Type | Polyclonal Antibody |
Application Notes | This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. |
Base Catalog Number | 56960 |
Concentration | Active Antibody: 1.0 mg/ml |
Conjugate | Unconjugated |
Format | lyophilized |
Grade | Molecular Grade |
Host | Goat |
Pack Size | 1 mg |
Solubility | Soluble in water |
Species Reactivity | Human |
Specificity | The total protein is measured by absorbance (A280nm). Antibody titer is standardized by equivalence-point precipitation. Each affinity purified F(ab')2 fragment is tested for purity at 10 mg/ml using immunoelectrophoresis. The product is goat F(ab')2; no trace of albumin; whole molecule IgG or Fc is detected. It shows reactivity to human IgM; cross-reactivity to other species may exist. Antibody activity to human IgG; IgA; or non-immunoglobulin serum proteins is not present. |
Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |