Goat IgG Fraction To ß-Galactosidase (No Cross To E. coli)
Product is goat IgG fraction to ß-galactosidase (no cross to E. coli) and buffer salts.
Goat IgG fraction to β-Galactosidase is used in enzyme Immunoassays, fluorescein Immunoassays, blots, cell or tissue immunostaining. Gives sharp precipitation lines in IEP and ID techniques. Suitable for conjugation with enzymes, radiolabels, fluorochromes, or other markers; suitable for attachment to solid supports for use as an immunoadsorbant. It is specially prepared for the detection and purification of recombinant proteins.
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunoassays | Immunoelectrophoresis | Immunodiffusion | Immunoprecipitation
|Analysis Note||The total protein is measured by using the Biuret procedure, with bovine albumin as a standard. Antibody titer is standardized by equivalence-point precipitation. The IgG fraction is tested for purity at 20 mg/mL using immunoelectrophoresis; No trace of albumin is detected.|
|Antibody Type||Polyclonal Antibody|
|Application Notes||Goat IgG fraction to β-Galactosidase is used in enzyme Immunoassays, fluorescein Immunoassays, blots, cell or tissue immunostaining. Gives sharp precipitation lines in IEP and ID techniques. Suitable for conjugation with enzymes, radiolabels, fluorochromes, or other markers; suitable for attachment to solid supports for use as an immunoadsorbant. It is specially prepared for the detection and purification of recombinant proteins.|
|Concentration||Total Protein: 31.1 mg/mL; Antibody Protein: 3 mg/mL; Conc/Titer: 1:50-1:200 Tissue Stain; 1:100-1:500 Immunoblot; 1:400-1:2;000 ELISA; conc is at 3 mg/mL|
|Immunogen||Recombinant full length protein of <i>Escherichia coli</i> β Galactosidase.|
|Applications||Immunoassays | Immunoelectrophoresis | Immunodiffusion | Immunoprecipitation|
|Pack Size||1 mL|
|Preparation Method||The antibody is produced by hyperimmunizing goats with the enzyme β-galactosidase from <i>Escherichia coli</i>. The IgG fraction is prepared by delipidation, ammonium sulfate fractionation, and anion exchange chromatography, and is further purified by solid-phase absorption to remove antibodies to non-β-galactosidase <i>E. coli</i> proteins. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 μm filter, and vialed. No preservative is added.|
|Solubility||Soluble in water|
|Specificity||It shows reactivity to mammalian beta-galactosidase and is tested by Western or dot blot to ensure monospecificity using lysates of D-lac and strain 3.3 of <i>E. coli</i>.|
|Storage and Handling||Store unopened product at 2-8°C. For long term storage; aliquot and freeze at -20°C. Avoid repeated freezing and thawing.|
|Typical Working Concentration||Suggested working dilution: 1:50 - 1:200 for Tissue Staining; 1:100 - 1:500 for Immunoblotting; 1:400 - 2,000 for Elisa|
|Uniprot Number||SwissProt: P00722 <i>Escherichia coli</i>|