Goat IgG Fraction To ß-Galactosidase (No Cross To E. coli)

Goat IgG Fraction To ß-Galactosidase (No Cross To E. coli)

Packaging size: 1 mL

SKU 0856028
Price:
$245.70
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Description

Product Description

Product is goat IgG fraction to ß-galactosidase (no cross to E. coli) and buffer salts.

Application Notes

Goat IgG fraction to β-Galactosidase is used in enzyme Immunoassays, fluorescein Immunoassays, blots, cell or tissue immunostaining. Gives sharp precipitation lines in IEP and ID techniques. Suitable for conjugation with enzymes, radiolabels, fluorochromes, or other markers; suitable for attachment to solid supports for use as an immunoadsorbant. It is specially prepared for the detection and purification of recombinant proteins.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunoassays | Immunoelectrophoresis | Immunodiffusion | Immunoprecipitation

Specifications
SKU 0856028
Analysis Note The total protein is measured by using the Biuret procedure, with bovine albumin as a standard. Antibody titer is standardized by equivalence-point precipitation. The IgG fraction is tested for purity at 20 mg/mL using immunoelectrophoresis; No trace of albumin is detected.
Antibody Category Polyclonal
Antibody Type Polyclonal Antibody
Application Notes Goat IgG fraction to β-Galactosidase is used in enzyme Immunoassays, fluorescein Immunoassays, blots, cell or tissue immunostaining. Gives sharp precipitation lines in IEP and ID techniques. Suitable for conjugation with enzymes, radiolabels, fluorochromes, or other markers; suitable for attachment to solid supports for use as an immunoadsorbant. It is specially prepared for the detection and purification of recombinant proteins.
Concentration Total Protein: 31.1 mg/mL; Antibody Protein: 3 mg/mL; Conc/Titer: 1:50-1:200 Tissue Stain; 1:100-1:500 Immunoblot; 1:400-1:2;000 ELISA; conc is at 3 mg/mL
Conjugate Unconjugated
Formulation Details Liquid
Host Goat
Immunogen Recombinant full length protein of <i>Escherichia coli</i> β Galactosidase.
Applications Immunoassays | Immunoelectrophoresis | Immunodiffusion | Immunoprecipitation
Pack Size 1 mL
Preparation Method The antibody is produced by hyperimmunizing goats with the enzyme β-galactosidase from <i>Escherichia coli</i>. The IgG fraction is prepared by delipidation, ammonium sulfate fractionation, and anion exchange chromatography, and is further purified by solid-phase absorption to remove antibodies to non-β-galactosidase <i>E. coli</i> proteins. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 μm filter, and vialed. No preservative is added.
Presentation Liquid
Solubility Soluble in water
Specificity It shows reactivity to mammalian beta-galactosidase and is tested by Western or dot blot to ensure monospecificity using lysates of D-lac and strain 3.3 of <i>E. coli</i>.
Storage and Handling Store unopened product at 2-8°C. For long term storage; aliquot and freeze at -20°C. Avoid repeated freezing and thawing.
Trade Name Cappel™
Typical Working Concentration Suggested working dilution: 1:50 - 1:200 for Tissue Staining; 1:100 - 1:500 for Immunoblotting; 1:400 - 2,000 for Elisa
Uniprot Number SwissProt: P00722 <i>Escherichia coli</i>
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.