Rabbit IgG Fraction To Sheep Red Blood Cells

Rabbit IgG Fraction To Sheep Red Blood Cells

Packaging size: 2 mL

SKU 0855806
Price:
$211.05
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Description

Product Description

Product is the lyophilized powder of rabbit IgG fraction to sheep red blood cells and buffer salts.

Application Notes

Immunoblotting; Immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. This product is suitable for sensitizing cells in hemolytic complement assays, and for lymphocyte screening procedures, especially screening for T cells and T receptor-bearing lymphocytes. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers.

Usage Statement

This product is intended FOR RESEARCH USE ONLY, not for use in diagnostic or therapeutic procedures involving humans or animals. This is to certify that this product has been obtained from healthy animals at USDA-inspected facilities. The product is not hazardous and does not contain any live pathogen.

Key Applications

Immunoassays | immmunoelectrophoresis| Immunohistochemistry (Paraffin; acetone)

Specifications
SKU 0855806
Analysis Note The total protein is measured using the Biuret procedure, with bovine albumin as a standard. Hemagglutination titer is standardized with an in-house control by measurement of agglutination end-point with 10% sheep red blood cells. Each IgG fraction is tested for purity at a minimum of 40 mg/mL using immunoelectrophoresis. The product is mostly rabbit IgG; no trace of albumin is detected.
Antibody Category Polyclonal
Antibody Type Polyclonal Antibody
Application Notes Immunoblotting; Immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. This product is suitable for sensitizing cells in hemolytic complement assays, and for lymphocyte screening procedures, especially screening for T cells and T receptor-bearing lymphocytes. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers.
Concentration Total Protein: 20.5 mg/mL Hemagglutination Titer: 1:1600
Conjugate Unconjugated
Formulation Details Lyophilized
Host Rabbit
Applications Immunoassays | immmunoelectrophoresis| Immunohistochemistry (Paraffin; acetone)
Pack Size 2 mL
Physical Appearance Lyophilized powder
Preparation Method The antiserum is prepared by immunizing healthy rabbits with red blood cells obtained from healthy sheep. The IgG fraction is prepared from the specific rabbit antiserum by delipidation, ammonium sulfate fractionation and anion exchange chromatography. The resulting IgG fraction is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized.
Presentation Lyophilized (IgG)
Research Areas Blood Proteins
Solubility Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material
Species Reactivity Sheep
Specificity The total protein is measured using the Biuret procedure; with bovine albumin as a standard. Hemagglutination titer is standardized with an in-house control by measurement of agglutination end-point with 10% sheep red blood cells. Each IgG fraction is tested for purity at a minimum of 40 mg/ml using immunoelectrophoresis. The product is mostly rabbit IgG; no trace of albumin is detected.
Storage and Handling In lyophilized form; this product has a shelf life of at least five years from the date of manufacture when stored at 2-8°C. For long term storage; aliquot and store reconstituted product frozen at -20°C or lower for up to one year.
Trade Name Cappel™
Usage Statement This product is intended FOR RESEARCH USE ONLY, not for use in diagnostic or therapeutic procedures involving humans or animals. This is to certify that this product has been obtained from healthy animals at USDA-inspected facilities. The product is not hazardous and does not contain any live pathogen.