Peroxidase-Conjugated Goat IgG Fraction To Rat IgG (Whole Molecule)

Peroxidase-Conjugated Goat IgG Fraction To Rat IgG (Whole Molecule)

Packaging size: 2 mL

SKU 0855770
Price:
$187.15
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Description

Product Description

Peroxidase-Conjugated Goat igg Fraction to Rat igg (Whole Molecule) is the lyophilized powder of horseradish peroxidase (HRP)-conjugated goat IgG fraction to rat IgG (whole molecule) and buffer salts.

Application Notes

Peroxidase-Conjugated Goat igg Fraction to Rat igg (Whole Molecule) is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies or their fragments are recommended to avoid non-specific binding from inherent antibodies of host animals. This product is also used in immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunohistochemistry | Immunoblotting |ELISA

Specifications
SKU 0855770
Analysis Note The total protein is measured using the Biuret procedure with bovine albumin as the standard. To ensure that the product titer falls within the required range, antibody titer is standardized by microtiter plate ELISA with rat IgG. The product is tested for purity and specificity at final concentration by immunoelectrophoresis. The antibody is predominantly goat IgG; no trace of albumin is detected.
Antibody Category Polyclonal
Antibody Type Polyclonal Antibody
Application Notes Peroxidase-Conjugated Goat igg Fraction to Rat igg (Whole Molecule) is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. Note: F(ab')<sub>2</sub> fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies or their fragments are recommended to avoid non-specific binding from inherent antibodies of host animals. This product is also used in immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections.
Concentration Total Protein: 5.5 mg/mL Titer: 1:120;000
Conjugate HRP
Formulation Lyophilized
Host Goat
Applications Immunohistochemistry | Immunoblotting |ELISA
Pack Size 2 mL
Physical Appearance Lyophilized powder
Preparation Method Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.01% thimerosal, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized.
Presentation lyophilized
Product Families Description Peroxidase-Conjugated Goat igg Fraction to Rat igg (Whole Molecule) is the lyophilized powder of horseradish peroxidase (HRP)-conjugated goat IgG fraction to rat IgG (whole molecule) and buffer salts.
Purity Purified
Solubility Soluble in water
Species Reactivity Rat; (Whole Molecule)
Specificity It shows reactivity to rat IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to non-immunoglobulin serum proteins is not present.
Storage and Handling Store lyophilized product at 2-8°C. For long-term storage; aliquot and freeze reconstituted product at -20°C or lower.
Typical Working Concentration SUGGESTED WORKING DILUTIONS: 1:500 - 1:4,000 for Tissue Staining 1:1,000 - 1;8,000 for Immunoblotting 1:2,000 - 1:16,000 for Elisa
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.