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Goat Affinity Purified Antibody To Rat IgG (Whole Molecule)

Goat Affinity Purified Antibody To Rat IgG (Whole Molecule)

Packaging size: 2 mg

SKU 0855740
Price:
$152.00
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Description

Product Description

Goat affinity purified antibody to rat IgG (whole molecule) is goat affinity purified antibody to rat IgG (whole molecule) and buffer salts.

Application Notes

Goat affinity purified antibody to rat IgG (whole molecule) is used in immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. These affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals. Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunoassays | Immunohistochemistry | ELISA

Specifications
SKU 0855740
Analysis Note The total protein is measured by absorbance (A280 nm). Antibody titer is standardized by equivalence-point precipitation. Each affinity purified antibody is tested for purity at 10 mg/mL using immunoelectrophoresis. The product is goat IgG; no trace of albumin is detected.
Antibody Category Polyclonal
Antibody Type Polyclonal Antibody
Application Notes Goat affinity purified antibody to rat IgG (whole molecule) is used in immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. These affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals. Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.
Concentration Total Protein: 1.02 mg/mL Active Antibody: 1.0 mg/mL
Conjugate Unconjugated
Formulation Details Purified
Host Goat
Applications Immunoassays | Immunohistochemistry | ELISA
Pack Size 2 mg
Physical Appearance Liquid
Preparation Method The affinity purified antibody is prepared from the specific goat IgG fraction by solid phase absorption. The resulting affinity purified antibody is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 µm filter, and vialed. No preservative has been added.
Presentation Affinity purified liquid
Product Families Description Goat affinity purified antibody to rat IgG (whole molecule) is goat affinity purified antibody to rat IgG (whole molecule) and buffer salts.
Purity Affinity Purified
Recommended Replacement For 0855736 | 0855737 | 0867047
Solubility Soluble in water
Species Reactivity Rat; (Whole Molecule)
Specificity It shows reactivity to rat IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to non-immunoglobulin serum proteins is not present.
Storage and Handling Store unopened product at 2-8°C. Store opened material no longer than 1-2 days at 2-8°C unless preservative is added. For long term storage; aliquot and store with 40% glycerol (v/v) at -20°C.
Typical Working Concentration SUGGESTED WORKING DILUTIONS 1:50 - 1:200 for Tissue Staining 1:100 - 1:500 for Immunoblotting 1:400 - 1:2,000 for Elisa
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.