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Goat IgG Fraction To Rat Complement C3

Goat IgG Fraction To Rat Complement C3

Packaging size: 2 mL

SKU 0855730
Price:
$223.50
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Description

Product Description

Goat IgG Fraction To Rat Complement C3 is the lyophilized powder of goat IgG fraction to rat complement C3 and buffer salts.

Application Notes

Goat IgG Fraction To Rat Complement C3 is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. The product has been successfully used in Immunoblotting. It detects C3 from human, equine, hamster, Guinea pig, murine, rabbit, rat, pig, and non-human primate samples. It does not cross react wtih bovine, canine, chicken, feline and sheep samples. It has been successfully used in immunocytochemistry, Western blot, Immunoelectrophoresis, and flow cytometry applications. It detects C3 from human, equine, hamster, Guinea pig, murine, rabbit, rat, pig, and non-human primate samples. It does not cross react wtih bovine, canine, chicken, feline and sheep samples. It has been successfully used in immunocytochemistry, Western blot, Immunoelectrophoresis, and flow cytometry applications.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunoassays | Immunoelectrophoresis | Immunodiffusion | Immunoprecipitation

Specifications
SKU 0855730
Analysis Note The total protein is measured using the Biuret procedure, with bovine albumin as a standard. Antibody titer is standardized to an in-house standard by equivalence-point precipitation. Each IgG fraction is tested for purity and specificity at a minimum of 40 mg/mL using immunoelectrophoresis. The product is mostly goat IgG; no trace of albumin is detected.
Antibody Category Polyclonal
Antibody Type Polyclonal Antibody
Application Notes Goat IgG Fraction To Rat Complement C3 is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. The product has been successfully used in Immunoblotting. It detects C3 from human, equine, hamster, Guinea pig, murine, rabbit, rat, pig, and non-human primate samples. It does not cross react wtih bovine, canine, chicken, feline and sheep samples. It has been successfully used in immunocytochemistry, Western blot, Immunoelectrophoresis, and flow cytometry applications. It detects C3 from human, equine, hamster, Guinea pig, murine, rabbit, rat, pig, and non-human primate samples. It does not cross react wtih bovine, canine, chicken, feline and sheep samples. It has been successfully used in immunocytochemistry, Western blot, Immunoelectrophoresis, and flow cytometry applications.
Concentration Total Protein: 33.9 mg/mL Antibody Protein: 6 mg/mL
Conjugate Unconjugated
Formulation Details Lyophilized
Gene ID (Hu) 718, (Ms) 12266, (Rt) 24232
Host Goat
Immunogen Human complement C3
Applications Immunoassays | Immunoelectrophoresis | Immunodiffusion | Immunoprecipitation
Pack Size 2 mL
Physical Appearance Lyophilized powder
Preparation Method The IgG fraction is prepared from the specific goat antiserum by delipidation, ammonium sulfate fractionation and anion exchange chromatography. The resulting IgG fraction is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, 0.05% sodium azide, pH 7.3, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized.
Presentation Lyophilized (IgG)
Research Areas Complement
Solubility Soluble in water
Species Reactivity Rat
Specificity It shows reactivity to rat complement C3; cross-reactivity to other species may exist. Antibody activity to other serum proteins is not present.
Storage and Handling Store lyophilized product at 2-8°C. Store reconstituted material no longer than 1-2 days at 2-8°C. For long term storage; aliquot and store reconstituted product frozen at -20°C or lower.
Uniprot Number (Hu) P01024, (Ms) P01027, (Rt) P01026
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.