Dino

Goat Affinity Purified Antibody To Rabbit IgG (Whole Molecule)

Goat Affinity Purified Antibody To Rabbit IgG (Whole Molecule)

MDL Number: MFCD00162781

Packaging size: 2 mg

SKU 0855641
Price:
$192.05
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Description

Product Description

Product is goat affinity purified antibody to rabbit IgG (whole molecule) and buffer salts.

Application Notes

Goat affinity purified antibody to rabbit IgG (Whole molecule) is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques, for conjugation with enzymes, radiolabels, fluorochromes or other markers, attachment to solid supports for use as an immunoadsorbant and used as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. These affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors).

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunoassays | Precipitating antibody | Immunoblot

Specifications
SKU 0855641
Analysis Note The total protein is measured by absorbance (A280nm). Antibody titer is standardized by equivalence-point precipitation. Each affinity purified antibody is tested for purity at 10 mg/mL using immunoelectrophoresis. The product is goat IgG; no trace of albumin is detected. Antibody activity to non-immunoglobulin serum proteins is not present.
Antibody Category Polyclonal
Antibody Type Polyclonal Antibody
Application Notes Goat affinity purified antibody to rabbit IgG (Whole molecule) is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques, for conjugation with enzymes, radiolabels, fluorochromes or other markers, attachment to solid supports for use as an immunoadsorbant and used as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. These affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors).
Concentration Total Protein: 1.06 mg/mL; Active Antibody: 1.0 mg/mL
Conjugate Unconjugated
Formulation Details Purified
Host Goat
Immunogen Rabbit IgG
Applications Immunoassays | Precipitating antibody | Immunoblot
Pack Size 2 mg
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Physical Appearance Liquid
Preparation Method The affinity purified antibody is prepared from the specific goat IgG fraction by solid phase absorption. The resulting affinity purified antibody is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 μm filter, and vialed.
Presentation Affinity purified liquid
Product Families Description Product is goat affinity purified antibody to rabbit IgG (whole molecule) and buffer salts.
Purity Affinity Purified
Recommended Replacement For 867035
Solubility Soluble in 1 mL of 0.135 M sodium chloride.
Species Reactivity Rabbit; (Whole Molecule)
Specificity It shows reactivity to rabbit IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to non-immunoglobulin serum proteins is not present.
Storage and Handling Store unopened product at 2-8°C. For long term storage; aliquot and store with 40% glycerol (v/v) at -20°C.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.