Goat IgG Fraction To Rabbit IgG (Whole Molecule)

Goat IgG Fraction To Rabbit IgG (Whole Molecule)

Packaging size: 5 mL

SKU 0855622
Price:
$239.85
In Stock
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Description

Product Description

Product is the lyophilized powder of goat IgG fraction to rabbit IgG (whole molecule) and buffer salts.

Application Notes

This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. It is also used as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals).

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunoassays | ELISA | Immunohistochemistry | Immunoblot

Specifications
SKU 0855622
Analysis Note The total protein is measured using the Biuret procedure, with bovine albumin as a standard. Antibody titer is standardized to an in-house standard by equivalence-point precipitation. Each IgG fraction is tested for purity and specificity at a minimum of 40 mg/mL using immunoelectrophoresis. The product is mostly goat IgG; no trace of albumin is detected.
Antibody Category Polyclonal
Antibody Type Polyclonal Antibody
Application Notes This product is suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. It is also used as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. (Note: F(ab')<sub>2</sub> fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals).
Concentration Total Protein: 26.5 mg/mL; Antibody Protein: 12 mg/mL
Conjugate Unconjugated
Formulation Lyophilized
Host Goat
Applications Immunoassays | ELISA | Immunohistochemistry | Immunoblot
Pack Size 5 mL
Preparation Method The IgG fraction is prepared from the specific goat antiserum by delipidation, ammonium sulfate fractionation and anion exchange chromatography. The resulting IgG fraction is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized.
Presentation Lyophilized (IgG)
Recommended Replacement For 0855623 | 0855628 | 0855635 | 0865118
Solubility Soluble in water
Species Reactivity Rabbit; (Whole Molecule)
Specificity It shows reactivity to rabbit IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to non-immunoglobulin serum proteins is not present.
Storage and Handling Store lyophilized product at 2-8°C. Store reconstituted material no longer than 1-2 days at 2-8°C; unless preservative is added. For long term storage; aliquot and store reconstituted product frozen at -20°C or lower.
Trade Name Cappel™
Typical Working Concentration Suggested working dilutions: 1:50 - 1:200 for Tissue Staining, 1:100 - 1:500 for Immunoblotting, 1:400 - 1:2,000 for Elisa
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.