Peroxidase-Conjugated Sheep IgG Fraction To Mouse IgG (No Cross To Human)

Peroxidase-Conjugated Sheep IgG Fraction To Mouse IgG (No Cross To Human)

MDL Number: MFCD00162644

Packaging size: 2 mL

SKU 0855558
Price:
$300.05
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Description

Product Description

Product is the lyophilized powder of horseradish peroxidase (HRP)-conjugated sheep IgG fraction to mouse IgG (no cross to human) and buffer salts.

Application Notes

Peroxidase conjugated Sheep IgG fraction to mouse IgG is used as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies or their fragments are recommended to avoid non-specific binding from inherent antibodies of host animals). It may be used to detect and quantitate the level of IgG in mouse serum and biological fluids via chromogenic, chemoluminescent or fluorogenic immunochemical or immunohistochemical techniques. It may also be used as a secondary antibody in assays that use mouse IgG as the primary antibody. The product can also be used for direct ELISA.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunohistochemistry | Immunoblotting |ELISA

Specifications
SKU 0855558
Analysis Note The total protein is measured using the Biuret procedure with bovine albumin as the standard. To ensure that the product titer falls within the required range, antibody titer is standardized by microtiter plate ELISA with mouse IgG and human IgG. The product is tested for purity and specificity at final concentration by immunoelectrophoresis. The antibody is predominantly sheep IgG; no trace of albumin is detected.
Antibody Category Polyclonal
Antibody Type Polyclonal Antibody
Application Notes Peroxidase conjugated Sheep IgG fraction to mouse IgG is used as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. (Note: F(ab')<sub>2</sub> fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies or their fragments are recommended to avoid non-specific binding from inherent antibodies of host animals). It may be used to detect and quantitate the level of IgG in mouse serum and biological fluids via chromogenic, chemoluminescent or fluorogenic immunochemical or immunohistochemical techniques. It may also be used as a secondary antibody in assays that use mouse IgG as the primary antibody. The product can also be used for direct ELISA.
Concentration Total Protein: 14.9 mg/mL; Titer: 1:187;000
Conjugate HRP
Formulation Details Lyophilized
Host Sheep
Immunogen Purified mouse IgG
Applications Immunohistochemistry | Immunoblotting |ELISA
MDL # MFCD00162644
Pack Size 2 mL
Physical Appearance Lyophilized
Preparation Method Antibody and highly purified HRP (Rz >3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.01% thimerosal, adjusted to standard titer, filtered through a 0.22 μm filter, vialed and lyophilized.
Presentation lyophilized
Purity Immunoaffinity Chromatography 
Recommended Replacement For 855569
Solubility Soluble in water
Species Reactivity Mouse; (No Cross To Human)
Specificity Product shows reactivity to mouse IgG. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to human IgG or mouse non-immunoglobulin serum proteins is not present.
Storage and Handling Store lyophilized product at 2-8°C. For long-term storage; aliquot and freeze reconstituted product at -20°C or lower.
Trade Name Cappel™
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.