Peroxidase-Conjugated Goat IgG Fraction To Mouse Immunoglobulins (IgG, IgA, IgM)

Peroxidase-Conjugated Goat IgG Fraction To Mouse Immunoglobulins (IgG, IgA, IgM)

MDL Number: MFCD00162699

Packaging size: 2 mL

SKU 0855556
Price:
$248.00
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Description

Product Description

Product is the lyophilized powder of horseradish peroxidase (HRP)-conjugated goat IgG fraction to mouse immunoglobulins (IgG,IgA,IgM) and buffer salts.

Application Notes

Peroxidase conjugated Goat IgG fraction to mouse immunoglobulins(IgG, IgA, IgM) is used as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies or their fragments are recommended to avoid non-specific binding from inherent antibodies of host animals). Detection of antibodies in sera of T. gondii-infected mice was done by ELISA using peroxidase-conjugated anti-mouse polyvalent immunoglobulin antibody as the secondary.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunohistochemistry | Immunoblotting |ELISA

Specifications
SKU 0855556
Analysis Note The total protein is measured using the Biuret procedure with bovine albumin as the standard. To ensure that the product titer falls within the required range, antibody titer is standardized by microtiter plate ELISA with mouse gamma globulin. The product is tested for purity and specificity at final concentration by immunoelectrophoresis. The antibody is predominantly goat IgG; no trace of albumin is detected.
Antibody Category Polyclonal
Antibody Type Polyclonal Antibody
Application Notes Peroxidase conjugated Goat IgG fraction to mouse immunoglobulins(IgG, IgA, IgM) is used as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. (Note: F(ab')<sub>2</sub> fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies or their fragments are recommended to avoid non-specific binding from inherent antibodies of host animals). Detection of antibodies in sera of T. gondii-infected mice was done by ELISA using peroxidase-conjugated anti-mouse polyvalent immunoglobulin antibody as the secondary.
Concentration Total Protein: 11.4 mg/mL; Titer: 1:200;000
Conjugate HRP
Formulation Lyophilized
Host Goat
Applications Immunohistochemistry | Immunoblotting |ELISA
MDL # MFCD00162699
Pack Size 2 mL
Physical Appearance Lyophilized powder
Preparation Method Antibody and highly purified HRP (Rz >3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 μm filter, vialed and lyophilized. No preservative has been added.
Presentation lyophilized
Purity Purified
Solubility Soluble in water
Species Reactivity Mouse; (IgG; IgA; IgM)
Specificity Product shows reactivity to mouse immunoglobulins (IgG;IgA;IgM); cross-reactivity to other species may exist. Antibody activity to non-immunoglobulin serum proteins is not present.
Storage and Handling Store lyophilized product at 2-8°C. For long-term storage; aliquot and freeze reconstituted product at -20°C or lower.
Trade Name Cappel™
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.