Peroxidase-Conjugated Goat IgG Fraction To Mouse IgG (Whole Molecule)

Peroxidase-Conjugated Goat IgG Fraction To Mouse IgG (Whole Molecule)

MDL Number: MFCD00162644

Packaging size: 2 mL

SKU 0855550
Price:
$271.15
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Description

Product Description

Product is the lyophilized powder of horseradish peroxidase (HRP)-conjugated goat IgG fraction to mouse IgG (whole molecule) and buffer salts. Anti-Mouse IgG is developed in goat using purified mouse IgG as the immunogen. Affinity isolation removes essentially all goat serum proteins, including immunoglobulins which do not specifically bind to mouse IgG.

Application Notes

This product is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies or their fragments are recommended to avoid non-specific binding from inherent antibodies of host animals).

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunohistochemistry | Immunoblotting |ELISA

Specifications
SKU 0855550
Analysis Note The total protein is measured using the Biuret procedure with bovine albumin as the standard. To ensure that the product titer falls within the required range, antibody titer is standardized by microtiter plate ELISA with mouse IgG. The product is tested for purity and specificity at final concentration by immunoelectrophoresis. The antibody is predominantly goat IgG; no trace of albumin is detected.
Antibody Category Polyclonal
Antibody Type Polyclonal Antibody
Application Notes This product is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining. (Note: F(ab')<sub>2</sub> fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies or their fragments are recommended to avoid non-specific binding from inherent antibodies of host animals).
Concentration Total Protein: 6.2 mg/ml Titer: 1:177;500
Conjugate HRP
Formulation Lyophilized
Host Goat
Immunogen Purified mouse IgG (whole molecule)
Applications Immunohistochemistry | Immunoblotting |ELISA
MDL # MFCD00162644
Pack Size 2 mL
Physical Appearance Lyophilized powder
Preparation Method Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized. No preservative has been added.
Presentation lyophilized
Product Families Description Product is the lyophilized powder of horseradish peroxidase (HRP)-conjugated goat IgG fraction to mouse IgG (whole molecule) and buffer salts. Anti-Mouse IgG is developed in goat using purified mouse IgG as the immunogen. Affinity isolation removes essentially all goat serum proteins, including immunoglobulins which do not specifically bind to mouse IgG.
Purity Purified
Solubility Soluble in water
Species Reactivity Mouse; (Whole Molecule)
Specificity It shows reactivity to mouse IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins. Antibody activity to non-immunoglobulin serum proteins is not present.
Storage and Handling Store lyophilized product at 2-8°C. For long-term storage; aliquot and freeze reconstituted product at -20°C or lower.
Trade Name Cappel™
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.