Dino

Fluorescein-Conjugated Goat F(Ab')2 Fragment To Mouse Complement C3

Fluorescein-Conjugated Goat F(Ab')2 Fragment To Mouse Complement C3

Packaging size: 2 mL

SKU 0855510
Price:
$343.95
In Stock
Request a quote
Description

Product Description

Product is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat F(ab')2 fragment to mouse complement C3 and buffer salts.

Application Notes

Fluorescein-Conjugated Goat F(ab')2 Fragment to Mouse Complement C3 is intended for laboratory use to qualitatively stain sections of frozen tissue, and is also suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunoassays | Protein Purification| Flow Cytometry (FACS) | ELISA | Immunohistochemistry (Paraffin) | Immunoblot

Specifications
SKU 0855510
Analysis Note The total protein is measured using the Biuret procedure with bovine albumin as the standard; F/P ratio is calculated using absorbance at 491.5nm. Antibody titer of the IgG fraction is standardized by equivalence-point precipitation. The F(ab')<sub>2</sub> fragments are tested for purity and specificity at a minimum of 20 mg/mL using immunoelectrophoresis; No trace of albumin, whole molecule IgG or Fc is detected. Antibody activity to other serum proteins is not present.
Antibody Category Polyclonal
Antibody Type Polyclonal Antibody
Application Notes Fluorescein-Conjugated Goat F(ab')2 Fragment to Mouse Complement C3 is intended for laboratory use to qualitatively stain sections of frozen tissue, and is also suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.
Concentration Total Protein: 6.9 mg/mL; Antibody Protein: 4 mg/mL
Conjugate FITC
Formulation Details Lyophilized
Host Goat
Applications Immunoassays | Protein Purification| Flow Cytometry (FACS) | ELISA | Immunohistochemistry (Paraffin) | Immunoblot
Pack Size 2 mL
Physical Appearance lyophilized powder
Preparation Method Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 μm filter, vialed, and lyophilized.
Presentation lyophilized
Purity Purified
Research Areas Complement
Solubility Soluble in water
Species Reactivity Mouse
Specificity The product is goat F(ab')2; no trace of albumin; whole molecule IgG or Fc is detected. This product shows reactivity to mouse complement C3; cross-reactivity to other species may exist. Antibody activity to other serum proteins is not present.
Storage and Handling Store lyophilized product at 2-8°C. Store reconstituted material no longer than 4 weeks at 2-8°C. For long-term storage; aliquot and freeze reconstituted product at -20°C or lower.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.