Fluorescein-Conjugated Goat IgG Fraction To Mouse Complement C3

Fluorescein-Conjugated Goat IgG Fraction To Mouse Complement C3

Packaging size: 2 mL

SKU 0855500
Price:
$245.70
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Description

Product Description

Product is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat IgG fraction to mouse complement C3 and buffer salts.

Application Notes

Fluorescein-Conjugated Goat IgG to Mouse Complement C3 is intended for laboratory use to qualitatively stain sections of frozen tissue, suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunoassays | Immunofluorescence assays (IFA) | Immunoblot

Specifications
SKU 0855500
Analysis Note The total protein is measured using the Biuret procedure with bovine albumin as the standard; F/P ratio is calculated using absorbance at 491.5nm. Antibody titer of the IgG fraction is standardized by equivalence-point precipitation. The IgG fraction is tested for purity and specificity at 40 mg/mL using immunoelectrophoresis; No trace of albumin is detected. Antibody activity to other serum proteins is not present.
Antibody Category Polyclonal
Antibody Type Polyclonal Antibody
Application Notes Fluorescein-Conjugated Goat IgG to Mouse Complement C3 is intended for laboratory use to qualitatively stain sections of frozen tissue, suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. F(ab')<sub>2</sub> fragments are recommended for staining of cells or tissues which contain Fc receptors.
Concentration Total Protein: 8.3 mg/mL; Antibody Protein: 4 mg/mL
Conjugate FITC
Formulation Details Lyophilized
Host Goat
Applications Immunoassays | Immunofluorescence assays (IFA) | Immunoblot
Pack Size 2 mL
Physical Appearance lyophilized powder
Preparation Method Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 μm filter, vialed, and lyophilized.
Presentation lyophilized
Purity Purified
Research Areas Complement
Solubility Soluble in water
Species Reactivity Mouse
Specificity The IgG fraction is tested for purity and specificity at 40 mg/ml using immunoelectrophoresis. The product is mostly goat IgG; no trace of albumin is detected. This product shows reactivity to mouse complement C3; cross-reactivity to other species may exist. Antibody activity to other serum proteins is not present.
Storage and Handling Store lyophilized product at 2-8°C. Store reconstituted material no longer than 4 weeks at 2-8°C. For long-term storage; aliquot and freeze reconstituted product at -20°C or lower.
Trade Name Cappel™
Typical Working Concentration Suggested working dilutions: 1:100 - 1:400 for Tissue Staining; 1:250 - 1:1,000 for Immunoblotting; 1:1,000 - 1:4,000 for ELISA
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.