Rabbit Affinity Purified Antibody To Mouse IgG (Whole Molecule)

Rabbit Affinity Purified Antibody To Mouse IgG (Whole Molecule)

Packaging size: 2 mg

SKU 0855480
Price:
$151.15
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Description

Product Description

Product is rabbit affinity purified antibody to mouse IgG (whole molecule) and buffer salts.

Application Notes

Rabbit Affinity Purified Antibody to Mouse IgG (Whole Molecule) is used as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. It is also suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. Used as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. These affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals. F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunoassays | Precipitating antibody |Immunoprecipitation

Specifications
SKU 0855480
Analysis Note The total protein is measured by absorbance (A<sub>280 nm</sub>). Antibody titer is standardized by equivalence-point precipitation. Each affinity purified antibody is tested for purity at 10 mg/mL using immunoelectrophoresis; No trace of albumin is detected. Antibody activity to non-immunoglobulin serum proteins is not present.
Antibody Category Polyclonal
Antibody Type Polyclonal Antibody
Application Notes Rabbit Affinity Purified Antibody to Mouse IgG (Whole Molecule) is used as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. It is also suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. Used as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. These affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals. F(ab')<sub>2</sub> fragments are recommended for staining of cells or tissues which contain Fc receptors.
Concentration Total Protein: 1.1 mg/mL; Active Antibody: 1.0 mg/mL
Conjugate Unconjugated
Formulation Details Liquid
Host Rabbit
Immunogen Mouse IgG
Isotype IgG
Applications Immunoassays | Precipitating antibody |Immunoprecipitation
Pack Size 2 mg
Preparation Method The affinity purified antibody is prepared from the specific rabbit IgG fraction by solid phase absorption. The resulting affinity purified antibody is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 µm filter, and vialed. No preservative has been added.
Presentation Affinity purified liquid
Purity Affinity Purified
Solubility Soluble in water
Species Reactivity Mouse; (Whole Molecule)
Specificity It shows reactivity to mouse IgG; cross-reactivity to other species may exist.
Storage and Handling Store unopened product at 2-8°C. For long term storage; aliquot and store with 40% glycerol (v/v) at -20°C.
Trade Name Cappel™
Typical Working Concentration Suggested working dilutions: 1:50 - 1:200 for Tissue Staining; 1:100 - 1:500 for Immunoblotting; 1:400 - 2,000 for ELISA
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.