Goat IgG Fraction To Mouse IgG (Whole Molecule)

Goat IgG Fraction To Mouse IgG (Whole Molecule)

MDL Number: MFCD00162638

Packaging size: 2 mL

SKU 0855455
Price:
$169.50
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Description

Product Description

Product is the lyophilized powder of goat IgG fraction to mouse IgG (whole molecule) and buffer salts.

Application Notes

Goat IgG fraction to mouse IgG (whole molecule) is used in immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. The antibody was used in ELISA and RIA in various studies. It is also suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals).

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed) | Immunofluorescence assays (IFA)

Specifications
SKU 0855455
Analysis Note The total protein is measured using the Biuret procedure, with bovine albumin as a standard. Antibody titer is standardized to an in-house standard by equivalence-point precipitation. Each IgG fraction is tested for purity and specificity at a minimum of 40 mg/mL using immunoelectrophoresis; No trace of albumin is detected. Antibody activity to non-immunoglobulin serum proteins is not present.
Antibody Category Polyclonal
Antibody Type Polyclonal Antibody
Application Notes Goat IgG fraction to mouse IgG (whole molecule) is used in immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. The antibody was used in ELISA and RIA in various studies. It is also suitable for use as a precipitating antibody. It gives sharp precipitin lines in immunoelectrophoresis and immunodiffusion techniques. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers, and for attachment to solid supports for use as an immunoadsorbant. This product is suitable for use as a primary reagent in enzyme immunoassays, fluorescein immunoassays and blot, cell or tissue immunostaining. (Note: F(ab')<sub>2</sub> fragments are recommended for staining of cells or tissues which contain Fc receptors. Affinity purified antibodies are recommended to avoid non-specific binding from the inherent antibodies of host animals).
Certifications The product is mostly goat IgG.
Concentration Total Protein: 24.6 mg/mL; Antibody Protein: 10 mg/mL
Conjugate Unconjugated
Formulation Lyophilized
Host Goat
Immunogen Pooled normal mouse serum
Applications Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed) | Immunofluorescence assays (IFA)
MDL # MFCD00162638
Pack Size 2 mL
Personal Protective Equipment Eyeshields, Gloves, Respirator filter
Physical Appearance Lyophilized powder
Preparation Method The IgG fraction is prepared from the specific goat antiserum by delipidation, ammonium sulfate fractionation and anion exchange chromatography. The resulting IgG fraction is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 μm filter, vialed and lyophilized.
Presentation Lyophilized IgG
Product Families Description Product is the lyophilized powder of goat IgG fraction to mouse IgG (whole molecule) and buffer salts.
Solubility Soluble in water
Species Reactivity Mouse; (Whole Molecule)
Specificity It shows reactivity to mouse IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins.
Storage and Handling Lyophilized product stored at 2-8°C has a shelf life of at least five years. After reconstitution; product will retain activity for at least 6 months stored at 2-8°C. For long term storage of up to two years; aliquot and store reconstituted product frozen at -20°C or lower.
Trade Name Cappel™
Typical Working Concentration Anti-Mouse IgG (whole molecule) antibody was used as secondary antibody in immunoblotting at a working dilution of 1:1000.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.