Fluorescein-Conjugated Goat IgG Fraction To Guinea Pig Complement C3
Product is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat IgG fraction to guinea pig complement C3 and buffer salts.
Fluorescein-Conjugated Goat IgG Fraction to Guinea Pig Complement C3 is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. This product is especially useful in anti-complement immunofluorescent (ACIF) assays.
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunoassays | Protein Purification| Flow Cytometry (FACS) | ELISA | Immunohistochemistry (Paraffin) | Immunoblot
|Alternate Names||(Anti-C3); Anti-Guinea Pig|
|Analysis Note||The total protein is measured using the Biuret procedure with bovine albumin as the standard; F/P ratio is calculated using absorbance at 491.5nm. Antibody titer of the IgG fraction is standardized with an in-house control by immunoelectrophoresis. The IgG fraction is tested for purity and specificity at 40 mg/mL using immunoelectrophoresis; No trace of albumin is detected. Antibody activity to serum albumin is not present.|
|Antibody Type||Polyclonal Antibody|
|Application Notes||Fluorescein-Conjugated Goat IgG Fraction to Guinea Pig Complement C3 is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. This product is especially useful in anti-complement immunofluorescent (ACIF) assays.|
|Concentration||Total Protein: 16.3 mg/mL|
|Immunogen||Guinea Pig Complement (C3)|
|Applications||Immunoassays | Protein Purification| Flow Cytometry (FACS) | ELISA | Immunohistochemistry (Paraffin) | Immunoblot|
|Pack Size||2 mL|
|Physical Appearance||Lyophilized powder|
|Preparation Method||Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 µm filter, vialed, and lyophilized.|
|Recommended Replacement For||0855383 | 0855393|
|Solubility||Soluble in water|
|Specificity||This product shows reactivity to guinea pig complement C3; cross-reactivity to other species may exist. Monospecific for the third component; of guinea pig complement (C3).|
|Storage and Handling||Store lyophilized product at 2-8°C. In the lyophilized state; the product has a minimum expiration period of three years from the date of manufacture. Store reconstituted material no longer than 4 weeks at 2-8°C. After reconstitution; the product can be aliquoted and stored at -20°C or lower for up to one year.|
|Typical Working Concentration||Suggested working dilution: Anti-complement immunofluorescent (ACIF) assays: 1:15|