Fluorescein-Conjugated Goat IgG Fraction To Guinea Pig Complement C3

Fluorescein-Conjugated Goat IgG Fraction To Guinea Pig Complement C3

Synonyms (Anti-C3); Anti-Guinea Pig

Packaging size: 2 mL

SKU 0855385
Price:
$263.30
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Description

Product Description

Product is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat IgG fraction to guinea pig complement C3 and buffer salts.

Application Notes

Fluorescein-Conjugated Goat IgG Fraction to Guinea Pig Complement C3 is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. This product is especially useful in anti-complement immunofluorescent (ACIF) assays.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunoassays | Protein Purification| Flow Cytometry (FACS) | ELISA | Immunohistochemistry (Paraffin) | Immunoblot

Specifications
SKU 0855385
Alternate Names (Anti-C3); Anti-Guinea Pig
Analysis Note The total protein is measured using the Biuret procedure with bovine albumin as the standard; F/P ratio is calculated using absorbance at 491.5nm. Antibody titer of the IgG fraction is standardized with an in-house control by immunoelectrophoresis. The IgG fraction is tested for purity and specificity at 40 mg/mL using immunoelectrophoresis; No trace of albumin is detected. Antibody activity to serum albumin is not present.
Antibody Category Polyclonal
Antibody Type Polyclonal Antibody
Application Notes Fluorescein-Conjugated Goat IgG Fraction to Guinea Pig Complement C3 is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. This product is especially useful in anti-complement immunofluorescent (ACIF) assays.
Concentration Total Protein: 16.3 mg/mL
Conjugate FITC
Formulation Details Lyophilized
Host Goat
Immunogen Guinea Pig Complement (C3)
Applications Immunoassays | Protein Purification| Flow Cytometry (FACS) | ELISA | Immunohistochemistry (Paraffin) | Immunoblot
Pack Size 2 mL
Physical Appearance Lyophilized powder
Preparation Method Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 µm filter, vialed, and lyophilized.
Presentation lyophilized
Purity Purified
Recommended Replacement For 0855383 | 0855393
Research Areas Complement
Solubility Soluble in water
Species Reactivity Pig
Specificity This product shows reactivity to guinea pig complement C3; cross-reactivity to other species may exist. Monospecific for the third component; of guinea pig complement (C3).
Storage and Handling Store lyophilized product at 2-8°C. In the lyophilized state; the product has a minimum expiration period of three years from the date of manufacture. Store reconstituted material no longer than 4 weeks at 2-8°C. After reconstitution; the product can be aliquoted and stored at -20°C or lower for up to one year.
Trade Name Cappel™
Typical Working Concentration Suggested working dilution: Anti-complement immunofluorescent (ACIF) assays: 1:15
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.