Peroxidase-Conjugated Rabbit IgG Fraction To Bovine Albumin

Peroxidase-Conjugated Rabbit IgG Fraction To Bovine Albumin

Packaging size: 2 mL

SKU 0855285
Price:
$962.05
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Description

Product Description

Product is the lyophilized powder of horseradish peroxidase (HRP)-conjugated rabbit IgG fraction to bovine albumin and buffer salts.

Application Notes

Peroxidase-Conjugated Rabbit IgG Fraction To Bovine Albumin is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunoassays | ELISA | Immunoblot | Immunohistochemistry | EM-Immunohistochemistry

Specifications
SKU 0855285
Analysis Note The total protein is measured using the Biuret procedure with bovine albumin as the standard. To ensure that the product titer falls within the required range, antibody titer is standardized by microtiter plate ELISA with bovine albumin. The product is tested for purity and specificity at final concentration by immunoelectrophoresis; No trace of albumin is detected. Antibody activity to other serum proteins is not present.
Antibody Category Polyclonal
Antibody Type Polyclonal Antibody
Application Notes Peroxidase-Conjugated Rabbit IgG Fraction To Bovine Albumin is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining.
Certifications The antibody is predominantly rabbit IgG.
Concentration Total Protein: 28.4 mg/mL; Titer: 1:110;000
Conjugate HRP
Formulation Details Lyophilized
Host Rabbit
Immunogen Albumin
Isotype IgG
Applications Immunoassays | ELISA | Immunoblot | Immunohistochemistry | EM-Immunohistochemistry
Pack Size 2 mL
Physical Appearance lyophilized powder
Preparation Method Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized.
Presentation Lyophilized PBS with 0.01% Thiomersal
Purity Purified
Solubility Soluble in water
Species Reactivity Cat; Bovine albumin
Specificity Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Albumin [Bovine Serum]
Storage and Handling Store lyophilized product at 2-8°C. For long-term storage; aliquot and freeze reconstituted product at -20°C or lower.
Typical Working Concentration ELISA: 1/2000 - 1/10000
Uniprot Number SwissProt: P02769 Cow
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.