Peroxidase-Conjugated Rabbit IgG Fraction To Bovine Albumin
Product is the lyophilized powder of horseradish peroxidase (HRP)-conjugated rabbit IgG fraction to bovine albumin and buffer salts.
Peroxidase-Conjugated Rabbit IgG Fraction To Bovine Albumin is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining.
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Immunoassays | ELISA | Immunoblot | Immunohistochemistry | EM-Immunohistochemistry
|Analysis Note||The total protein is measured using the Biuret procedure with bovine albumin as the standard. To ensure that the product titer falls within the required range, antibody titer is standardized by microtiter plate ELISA with bovine albumin. The product is tested for purity and specificity at final concentration by immunoelectrophoresis; No trace of albumin is detected. Antibody activity to other serum proteins is not present.|
|Antibody Type||Polyclonal Antibody|
|Application Notes||Peroxidase-Conjugated Rabbit IgG Fraction To Bovine Albumin is suitable for use as a reagent in enzyme immunoassays (EIA), cell and tissue staining (for light microscopy), cell and tissue labeling (for electron microscopy), and blot immunostaining.|
|Certifications||The antibody is predominantly rabbit IgG.|
|Concentration||Total Protein: 28.4 mg/mL; Titer: 1:110;000|
|Applications||Immunoassays | ELISA | Immunoblot | Immunohistochemistry | EM-Immunohistochemistry|
|Pack Size||2 mL|
|Physical Appearance||lyophilized powder|
|Preparation Method||Antibody and highly purified HRP (Rz>3.0) are conjugated under defined conditions to obtain optimally labeled product. Conjugated protein is purified by salt fractionation. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized.|
|Presentation||Lyophilized PBS with 0.01% Thiomersal|
|Solubility||Soluble in water|
|Species Reactivity||Cat; Bovine albumin|
|Specificity||Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Albumin [Bovine Serum]|
|Storage and Handling||Store lyophilized product at 2-8°C. For long-term storage; aliquot and freeze reconstituted product at -20°C or lower.|
|Typical Working Concentration||ELISA: 1/2000 - 1/10000|
|Uniprot Number||SwissProt: P02769 Cow|