Dino

Fluorescein-Conjugated Goat Affinity Purified Antibody To Human IgG (Whole Molecule)

Fluorescein-Conjugated Goat Affinity Purified Antibody To Human IgG (Whole Molecule)

Packaging size: 2 mg

SKU 0855197
Price:
$168.25
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Description

Product Description

Fluorescein-Conjugated Goat Affinity Purified Antibody to Human IgG (Whole Molecule) is fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat affinity purified antibody to human IgG (whole molecule) and buffer salts.

Application Notes

Fluorescein-Conjugated Goat Affinity Purified Antibody to Human IgG (Whole Molecule) is suitable for use in direct immunofluorescence, as well as for ANA (Anti-Nuclear Antibody)-indirect immunofluorescence. The antibody may also be used in FACS-based assays. It is also suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections, and blot immunostaining. Use of these affinity purified antibodies is recommended to avoid non-specific binding from the inherent antibodies of host animals. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors).

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS)

Specifications
SKU 0855197
Analysis Note The total protein is measured by absorbance (A280nm); F/P ratio is calculated using absorbance at 491.5nm. Antibody titer of the affinity purified antibody is standardized by equivalence-point precipitation. The affinity purified antibody is tested for purity at 10 mg/mL using immunoelectrophoresis.
Antibody Category Polyclonal
Antibody Type Polyclonal Antibody
Application Notes Fluorescein-Conjugated Goat Affinity Purified Antibody to Human IgG (Whole Molecule) is suitable for use in direct immunofluorescence, as well as for ANA (Anti-Nuclear Antibody)-indirect immunofluorescence. The antibody may also be used in FACS-based assays. It is also suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections, and blot immunostaining. Use of these affinity purified antibodies is recommended to avoid non-specific binding from the inherent antibodies of host animals. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors).
Certifications The product is goat IgG.
Concentration Total Protein: 1.12 mg/mL; Active Antibody: 1.0 mg/mL
Conjugate FITC
Formulation Details Liquid
Host Goat
Immunogen Pooled normal human serum
Applications Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS)
Pack Size 2 mg
Personal Protective Equipment Eyeshields, Gloves, half-mask respirator, multi-purpose combination respirator cartridge
Preparation Method Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide and 10% glycerol (v/v), adjusted to standard titer, filtered through a 0.22 µm filter, and vialed.
Presentation Liquid
Product Families Description Fluorescein-Conjugated Goat Affinity Purified Antibody to Human IgG (Whole Molecule) is fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat affinity purified antibody to human IgG (whole molecule) and buffer salts.
Purity Affinity Purified
Solubility Soluble in Water
Species Reactivity Human; (Whole Molecule)
Specificity This product shows reactivity to human IgG; cross-reactivity to other species may exist. Because the product is directed against whole IgG; some antibodies are expected to bind light chain sites common to all immunoglobulins.
Storage and Handling Store unopened product at 2-8°C. Store opened material no longer than 4 weeks at 2-8°C. For long term storage; aliquot and store with 40% glycerol (v/v) at -20°C.
Typical Working Concentration ANA (Anti-Nuclear Antibody)-indirect immunofluorescence: 1:32 or 1:64; Direct immunofluorescence: 1:16 . Dilution recommended: 1:100 - 1:400 for Tissue Staining; 1:250 - 1:1,000 for Immuno Blotting; 1:1,000 - 1:4,000 for ELISA/FIA
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.