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Fluorescein-Conjugated Goat F(Ab')2 Fragment To Human Complement C3

Fluorescein-Conjugated Goat F(Ab')2 Fragment To Human Complement C3

Synonyms Anti-C3; AHUS5; ARMD9; ASP; CPAMD1

Packaging size: 2 mL

SKU 0855191
Price:
$310.00
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Description

Product Description

Fluorescein-Conjugated Goat F(ab')2 Fragment to Human Complement C3 is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat F(ab')2 fragment to human complement C3 and buffer salts.

Application Notes

Fluorescein-Conjugated Goat F(ab')2 Fragment to Human Complement C3 has been successfully used in immunohistochemistry (frozen) applications. It is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. It has also been used in flow cytometry studies. It has been used to assess the binding of complement factors.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunoassays | Protein Purification| Flow Cytometry (FACS) | ELISA | Immunohistochemistry (Paraffin) | Immunoblot

Specifications
SKU 0855191
Analysis Note The total protein is measured using the Biuret procedure with bovine albumin as the standard; F/P ratio is calculated using absorbance at 491.5nm. Antibody titer of the IgG fraction is standardized by equivalence-point precipitation. The F(ab')<sub>2</sub> fragments are tested for purity and specificity at a minimum of 20 mg/mL using immunoelectrophoresis.
Antibody Category Polyclonal
Antibody Type Polyclonal Antibody
Application Notes Fluorescein-Conjugated Goat F(ab')2 Fragment to Human Complement C3 has been successfully used in immunohistochemistry (frozen) applications. It is suitable for use as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors. It has also been used in flow cytometry studies. It has been used to assess the binding of complement factors.
Certifications The product is goat F(ab')<sub>2</sub>.
Concentration Total Protein: 16.5 mg/mL; Antibody Protein: 7 mg/mL
Conjugate FITC
Formulation Details Lyophilized
Host Goat
Immunogen Human C3 purified from serum
Applications Immunoassays | Protein Purification| Flow Cytometry (FACS) | ELISA | Immunohistochemistry (Paraffin) | Immunoblot
Pack Size 2 mL
Preparation Method Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 µm filter, vialed, and lyophilized.
Presentation lyophilized
Purity Purified
Research Areas Complement
Solubility Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.
Species Reactivity human
Specificity This product shows reactivity to human complement C3; cross-reactivity to other species may exist.
Storage and Handling Store lyophilized product at 2-8°C. Store reconstituted material no longer than 4 weeks at 2-8°C. For long-term storage; aliquot and freeze reconstituted product at -20°C or lower.
Typical Working Concentration Immunohistochemistry (Frozen) (IHC (F)) 1:50 (dilution). Dilution Recommended: 1:100 - 1:400 for Tissue Staining; 1:250 - 1:1,000 for Immuno Blotting; 1:1,000 - 1:4,000 for ELISA/FIA
Uniprot Number (Hu) P01024
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.