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Fluorescein-Conjugated Goat IgG Fraction To Human Fibrinogen

Fluorescein-Conjugated Goat IgG Fraction To Human Fibrinogen

Packaging size: 2 mL

SKU 0855169
Price:
$305.00
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Description

Product Description

Fluorescein-conjugated Goat IgG Fraction to Human Fibrinogen is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat IgG fraction to human fibrinogen and buffer salts.

Application Notes

Fluorescein-conjugated Goat IgG Fraction to Human Fibrinogen is used as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS)

Specifications
SKU 0855169
Analysis Note The total protein is measured using the Biuret procedure with bovine albumin as the standard; F/P ratio is calculated using absorbance at 491.5nm. Antibody titer of the IgG fraction is standardized with an in-house control by immunoelectrophoresis. The IgG fraction is tested for purity and specificity at 40 mg/mL using immunoelectrophoresis. The product is mostly goat IgG; no trace of albumin is detected.
Antibody Category Polyclonal
Antibody Type Polyclonal Antibody
Application Notes Fluorescein-conjugated Goat IgG Fraction to Human Fibrinogen is used as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining. Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors.
Concentration Total Protein: 16.8 mg/mL; Antibody Protein: 3.5 mg/mL
Conjugate FITC
Formulation Details Lyophilized
Gene Symbol and Aliases FGA; MGC119423; MGC119425; Fib2; MGC119422
Host Goat
Immunogen Purified fibrinogen derived from pooled human plasma
Applications Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS)
Pack Size 2 mL
Preparation Method Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 μm filter, vialed, and lyophilized.
Presentation lyophilized
Recommended Replacement For 868294
Research Areas Atherosclerosis||Blood Proteins
Solubility Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.
Species Reactivity Human; IgG Fraction
Specificity This product shows reactivity to human fibrinogen; cross-reactivity to other species may exist. Antibody activity to serum proteins is not present.
Storage and Handling Store lyophilized product at 2-8°C for up to three years from the date of manufacture. Store reconstituted material no longer than 4 weeks at 2-8°C. For long-term storage; aliquot and freeze reconstituted product at -20°C or lower for up to two years.
Typical Working Concentration Dilution Recommendations: 1. Incident light excitation-Direct immunofluorescence: 1/10 - 1/80; Indirect immunofluorescence: 1/20 - 1/400. 2. Transmitted light excitation-Direct immunofluorescence: 1/5 - 1/40; Indirect immunofluorescence: 1/10 - 1/80; 1:100 - 1:400 for Tissue Staining; 1:250 - 1:1,000 for Immuno Blotting; 1:1,000 - 1:4,000 for ELISA/FIA
Uniprot Number P02671
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.