Fluorescein-Conjugated Goat IgG Fraction To Human Complement C1Q

Fluorescein-Conjugated Goat IgG Fraction To Human Complement C1Q

Synonyms C1QA antibody; C1QA Human antibody C1QB; C1QB antibody; C1QC antibody; Complement C1q subcomponent subunit A antibody Complement C1q subcomponent subunit B antibody; Complement C1q subcomponent subunit C antibody; complement component 1 q subcomponent A chain antibody; Complement component 1 q subcomponent alpha polypeptide antibody; complement component C1q A chain antibody; Complement component C1q B chain antibody; Complement component C1q C chain antibody

Packaging size: 2 mL

SKU 0855166
Price:
$489.45
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Description

Product Description

Fluorescein-conjugated Goat IgG Fraction to Human Complement C1q is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat IgG fraction to human complement C1q and buffer salts.

Application Notes

Fluorescein-conjugated Goat IgG Fraction to Human Complement C1q is used as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS)

Specifications
SKU 0855166
Alternate Names C1QA antibody; C1QA Human antibody C1QB; C1QB antibody; C1QC antibody; Complement C1q subcomponent subunit A antibody Complement C1q subcomponent subunit B antibody; Complement C1q subcomponent subunit C antibody; complement component 1 q subcomponent A chain antibody; Complement component 1 q subcomponent alpha polypeptide antibody; complement component C1q A chain antibody; Complement component C1q B chain antibody; Complement component C1q C chain antibody
Analysis Note The total protein is measured using the Biuret procedure with bovine albumin as the standard; F/P ratio is calculated using absorbance at 491.5nm. Antibody titer of the IgG fraction is standardized with an in-house control by immunoelectrophoresis. The IgG fraction is tested for purity and specificity at 40 mg/mL using immunoelectrophoresis. The product is mostly goat IgG; no trace of albumin is detected.
Antibody Category Polyclonal
Antibody Type Polyclonal Antibody
Application Notes Fluorescein-conjugated Goat IgG Fraction to Human Complement C1q is used as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining, and blot immunostaining.
Concentration Total Protein: 10.8 mg/mL
Conjugate FITC
Formulation Details Lyophilized
Host Goat
Immunogen C1q isolated from the normal human serum.
Applications Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS)
Pack Size 2 mL
Preparation Method Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 μm filter, vialed, and lyophilized.
Presentation lyophilized
Purity Purified
Research Areas Complement||Neuron marker
Solubility Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.
Species Reactivity Human; IgG Fraction
Specificity This product shows reactivity to human complement C1q; cross-reactivity to other species may exist. Antibody activity to other complement components or serum proteins is not present.
Storage and Handling Store lyophilized product at 2-8°C. Store reconstituted material no longer than 4 weeks at 2-8°C. For long-term storage; aliquot and freeze product at -20°C or lower.
Trade Name Cappel™
Typical Working Concentration Dilution Reccomondations: 1:100 - 1:400 for Tissue Staining; 1:250 - 1:1,000 for Immuno Blotting; 1:1,000 - 1:4,000 for ELISA/FIA
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.