Fluorescein-Conjugated Goat IgG Fraction To Human Albumin
Product Description
Fluorescein-Conjugated Goat IgG Fraction To Human Albumin is the lyophilized powder of fluorescein-5-isothiocyanate (FITC "Isomer I")-conjugated goat IgG fraction to human albumin and buffer salts.
Application Notes
Goat Anti-Human Albumin antibody is used as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining and blot immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors). It is also used in Immunoblot (1:200 to 1:80,000) ELISA (1:200 to 1:80,000) techniques.
Usage Statement
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications
Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS)
| SKU | 0855162 |
| Analysis Note | The total protein is measured using the Biuret procedure with bovine albumin as the standard; F/P ratio is calculated using absorbance at 491.5nm. Antibody titer of the IgG fraction is standardized by equivalence-point precipitation. The IgG fraction is tested for purity and specificity at 40 mg/mL using immunoelectrophoresis. The product is mostly goat IgG; no trace of albumin is detected. |
| Antibody Category | Polyclonal |
| Antibody Type | Polyclonal Antibody |
| Application Notes | Goat Anti-Human Albumin antibody is used as a reagent in immunofluorescence assays (IFA), cell staining (fluorescent microscopy and cell sorting), tissue staining and blot immunostaining. (Note: F(ab')2 fragments are recommended for staining of cells or tissues which contain Fc receptors). It is also used in Immunoblot (1:200 to 1:80,000) ELISA (1:200 to 1:80,000) techniques. |
| Concentration | Total Protein: 30.5 mg/mL; Antibody Protein: 8 mg/mL |
| Conjugate | FITC |
| Formulation Details | Lyophilized |
| Host | Goat |
| Immunogen | Human albumin |
| Applications | Immunoassays | Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen) | Immunofluorescence assays (IFA) | Flow Cytometry (FACS) |
| Pack Size | 2 mL |
| Physical Appearance | lyophilized powder |
| Preparation Method | Antibody and FITC are conjugated under defined conditions to obtain the specified molar ratio (3-6 mol/mol). Conjugated protein is purified by gel filtration. The product is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, with 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 μm filter, vialed, and lyophilized. |
| Presentation | lyophilized |
| Recommended Replacement For | 868290 |
| Research Areas | Immunoassays |
| Solubility | Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material. |
| Species Reactivity | Human; IgG Fraction |
| Specificity | This product shows reactivity to human albumin; cross-reactivity to other species may exist. Antibody activity to other serum proteins is not present. |
| Storage and Handling | Store lyophilized product at 2-8°C. Store reconstituted material no longer than 4 weeks at 2-8°C. For long-term storage; aliquot and freeze reconstituted product at -20°C or lower. |
| Typical Working Concentration | Dilution Recommendations: 1:100 - 1:400 for Tissue Staining; 1:250 - 1:1,000 for Immuno Blotting; 1:1,000 - 1:4,000 for ELISA/FIA |
| Usage Statement | Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |